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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2007.11.003
|2 doi
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|a pubmed24n0591.xml
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|a (DE-627)NLM17731155X
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|a (NLM)18242776
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
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|a Qi, Jin-Liang
|e verfasserin
|4 aut
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|a Expression analysis of light-regulated genes isolated from a full-length-enriched cDNA library of Onosma paniculatum cell cultures
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 22.04.2009
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Shikonin and its derivatives are formed in large amounts in dark-cultured Onosma paniculatum cells. In order to isolate and identify the genes regulating shikonin biosynthesis, we constructed and characterized a full-length-enriched cDNA library of dark-cultured cells by using the SMART (Switching Mechanism At 5'-end of RNA Transcript) cDNA synthesis and LD-PCR (long-distance PCR) strategies. The titer of the primary cDNA library was 1.04 x 10(6)pfu/mL with a recombination rate of 99.60%. Most of the cDNA inserts ranged from 1.0 to 2.5 kb, and 78.33% of the 76 randomly selected clones contained full-length coding regions. Expression analysis of randomly selected genes by small scale microarray revealed that 23 genes were down-regulated, including 17 genes with known functions, 2 genes with putative functions, and 4 novel genes, and that 3 genes were up-regulated (two-fold) in cells cultured under white light as compared with those cultured in the dark. Interestingly, two of the down-regulated genes, encoding aci-reductone dioxygenase (ARD)-like protein and ethylene responsive factor (ERF), are involved in ethylene biosynthesis and signal transduction, implying that ethylene might play an important role as a signal molecule in light-regulated shikonin formation. These data contribute to a better understanding of light-involvement in regulating the formation of plant secondary metabolites
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a DNA, Complementary
|2 NLM
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|a Ethylenes
|2 NLM
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| 650 |
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|a Naphthoquinones
|2 NLM
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| 650 |
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|a shikonin
|2 NLM
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| 650 |
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|a 3IK6592UBW
|2 NLM
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|a ethylene
|2 NLM
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|a 91GW059KN7
|2 NLM
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| 700 |
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|a Zhang, Wen-Ju
|e verfasserin
|4 aut
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|a Liu, Shao-Hua
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Hong
|e verfasserin
|4 aut
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| 700 |
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|a Sun, Deng-Yun
|e verfasserin
|4 aut
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| 700 |
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|a Xu, Guo-Hua
|e verfasserin
|4 aut
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| 700 |
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|a Shi, Ming-Wang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Zhi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Ming-Sheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Hui-Ming
|e verfasserin
|4 aut
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| 700 |
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|a Yang, Yong-Hua
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 165(2008), 14 vom: 29. Sept., Seite 1474-82
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:165
|g year:2008
|g number:14
|g day:29
|g month:09
|g pages:1474-82
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| 856 |
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|u http://dx.doi.org/10.1016/j.jplph.2007.11.003
|3 Volltext
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