In situ measurements of the dynamics of single giant DNA molecules at the toluene-trioctylamine/water interface by total internal reflection fluorescence microscopy
The dynamics of single giant deoxyribonucleic acid (T4GT7DNA, 165 600 base pairs) molecules was examined near and at the toluene/water, toluene-trioctylamine mixtures/water, and trioctylamine/water interfaces by total internal reflection fluorescence microscopy. The results were considerably affecte...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 24(2008), 5 vom: 04. März, Seite 1673-7 |
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| Weitere Verfasser: | , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2008
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Amines Water 059QF0KO0R Toluene 3FPU23BG52 DNA 9007-49-2 tri-N-octylamine |
| Zusammenfassung: | The dynamics of single giant deoxyribonucleic acid (T4GT7DNA, 165 600 base pairs) molecules was examined near and at the toluene/water, toluene-trioctylamine mixtures/water, and trioctylamine/water interfaces by total internal reflection fluorescence microscopy. The results were considerably affected by the trioctylamine content. With pure toluene or mixtures of lower trioctylamine volume contents (%VA), the randomly coiled DNA molecules diffused to near the interfaces. With mixtures of higher %VA (9 and 50%), the DNA molecules were stretched and adsorbed at the interfaces. There are a large number of anionic phosphate groups ((-O)2PO2-) in the DNA molecule that have an electrostatic affinity to protonated trioctylamine existing at the interface. In the case of pure trioctylamine, globular DNA molecules were adsorbed at the interface and also existed in the aqueous phase |
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| Beschreibung: | Date Completed 27.05.2008 Date Revised 24.11.2016 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la7027719 |