Functional PEG-modified thin films for biological detection

We report a general procedure to prepare functional organic thin films for biological assays on oxide surfaces. Silica surfaces were functionalized by self-assembly of an amine-terminated silane film using both vapor- and solution-phase deposition of 3'-aminopropylmethyldiethoxysilane (APMDES)....

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:	Langmuir : the ACS journal of surfaces and colloids. - 1992. - 24(2008), 5 vom: 04. März, Seite 2240-7
1. Verfasser:	Anderson, Aaron S (VerfasserIn)
Weitere Verfasser:	Dattelbaum, Andrew M, Montaño, Gabriel A, Price, Dominique N, Schmidt, Jurgen G, Martinez, Jennifer S, Grace, W Kevin, Grace, Karen M, Swanson, Basil I
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2008
Zugriff auf das übergeordnete Werk:	Langmuir : the ACS journal of surfaces and colloids
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Antigens, Bacterial Bacterial Toxins Silanes anthrax toxin Polyethylene Glycols 3WJQ0SDW1A

Beschreibung
Zusammenfassung:	We report a general procedure to prepare functional organic thin films for biological assays on oxide surfaces. Silica surfaces were functionalized by self-assembly of an amine-terminated silane film using both vapor- and solution-phase deposition of 3'-aminopropylmethyldiethoxysilane (APMDES). We found that vapor-phase deposition of APMDES under reduced pressure produced the highest quality monolayer films with uniform surface coverage, as determined by atomic force microscopy (AFM), ellipsometry, and contact angle measurements. The amine-terminated films were chemically modified with a mixture of carboxylic acid-terminated poly(ethylene glycol) (PEG) chains of varying functionality. A fraction of the PEG chains (0.1-10 mol %) terminated in biotin, which produced a surface with an affinity toward streptavidin. When used in pseudo-sandwich assays on waveguide platforms for the detection of Bacillus anthracis protective antigen (PA), these functional PEG surfaces significantly reduced nonspecific binding to the waveguide surface while allowing for highly specific binding. Detection of PA was used to validate these films for sensing applications in both buffer and complex media. Ultimately, these results represent a step toward the realization of a robust, reusable, and autonomous biosensor
Beschreibung:	Date Completed 27.05.2008 Date Revised 01.12.2018 published: Print-Electronic Citation Status MEDLINE
ISSN:	1520-5827
DOI:	10.1021/la7033438