Partial characterization and expression of leaf catalase in the CAM-inducible halophyte Mesembryanthemum crystallinum L

Catalase (CAT; EC 1.11.1.6) isolated from leaves of the halophytic plant Mesembryanthemum crystallinum is characterized by a high apparent molecular mass of about 320kDa, and high resistance to denaturing agents (10% ME). SDS-treatment breaks active oligomeric CAT into the less active and putatively...

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Détails bibliographiques
Publié dans:Plant physiology and biochemistry : PPB. - 1991. - 46(2008), 4 vom: 10. Apr., Seite 421-7
Auteur principal: Niewiadomska, Ewa (Auteur)
Autres auteurs: Miszalski, Zbigniew
Format: Article en ligne
Langue:English
Publié: 2008
Accès à la collection:Plant physiology and biochemistry : PPB
Sujets:Journal Article Research Support, Non-U.S. Gov't Protein Subunits Catalase EC 1.11.1.6
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520 |a Catalase (CAT; EC 1.11.1.6) isolated from leaves of the halophytic plant Mesembryanthemum crystallinum is characterized by a high apparent molecular mass of about 320kDa, and high resistance to denaturing agents (10% ME). SDS-treatment breaks active oligomeric CAT into the less active and putatively dimeric form of 160kDa apparent molecular mass. Three subunits are resolved after denaturing PAGE: 79, 74 and 62kDa. Higher molecular masses of subunits coincide with increased activity of CAT. M. crystallinum leaf CAT reveals a diel variation in the resistance to denaturing factors and the stability of CAT is increased in a light-dependent manner both in C(3)- and in CAM-induced plants. Unchanged level of leaf CAT transcripts is documented in the diurnal cycle of C(3) plants and after salinity-induced crassulacean acid metabolism (CAM) 
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