|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM176744916 |
| 003 |
DE-627 |
| 005 |
20231223144715.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231223s2007 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/erm307
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0589.xml
|
| 035 |
|
|
|a (DE-627)NLM176744916
|
| 035 |
|
|
|a (NLM)18182441
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a López-Ochoa, Luisa
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Structural relationships between diverse cis-acting elements are critical for the functional properties of a rbcS minimal light regulatory unit
|
| 264 |
|
1 |
|c 2007
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 28.04.2008
|
| 500 |
|
|
|a Date Revised 17.11.2011
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Light-regulation of photosynthesis-associated nuclear genes is mediated by multipartite cis-regulatory units located in their promoter regions. The combination, spacing, and relative orientation of transcription factor binding sites in these units influences the assembly of specific multiprotein complexes that control gene expression. In this work, the functional architecture of the conserved modular array 5 (CMA5), a previously characterized minimal light-regulatory unit of rbcS gene promoters, has been analysed. With the aim of defining the sequences of CMA5 that, besides the I- and G-box elements, are essential for CMA5 responsiveness to light and chloroplast-derived signals, a series of mutations affecting the sequences flanking these elements was performed. It was found that an I-box associated module, named IbAM5, is essential for CMA5 functionality and is able to bind nuclear proteins in vitro. The spacing requirements of the I- and G-box elements in achieving adequate combinatorial interaction were also studied as well as the effect of interchanging the relative position of these elements in the native rbcS promoter arrangement. The results show that helical phasing and distance between the I- and G-box motifs are critical to determine the functionality and transcriptional strength of CMA5. Furthermore, it is shown that the relative position of the IbAM5/I-box composite element and the G-box element is not critical for the enhancer activity of CMA5, as long as the proper distance between them is maintained. Taken together, these results suggest that the light-responsive, plastid-dependent activity of CMA5 requires the synergistic interaction of several DNA-binding transcription factors assembled in a higher-order nucleoprotein complex
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a DNA, Intergenic
|2 NLM
|
| 650 |
|
7 |
|a Ribulose-Bisphosphate Carboxylase
|2 NLM
|
| 650 |
|
7 |
|a EC 4.1.1.39
|2 NLM
|
| 700 |
1 |
|
|a Acevedo-Hernández, Gustavo
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Martínez-Hernández, Aída
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Argüello-Astorga, Gerardo
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Herrera-Estrella, Luis
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 58(2007), 15-16 vom: 12., Seite 4397-406
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:58
|g year:2007
|g number:15-16
|g day:12
|g pages:4397-406
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/erm307
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 58
|j 2007
|e 15-16
|b 12
|h 4397-406
|