|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM176513035 |
| 003 |
DE-627 |
| 005 |
20231223144215.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231223s2007 xx |||||o 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed24n0588.xml
|
| 035 |
|
|
|a (DE-627)NLM176513035
|
| 035 |
|
|
|a (NLM)18157808
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Sinha, Neeraj
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Tailoring 13C labeling for triple-resonance solid-state NMR experiments on aligned samples of proteins
|
| 264 |
|
1 |
|c 2007
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 29.04.2009
|
| 500 |
|
|
|a Date Revised 11.11.2023
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a 2007 John Wiley & Sons, Ltd
|
| 520 |
|
|
|a In order to develop triple-resonance solid-state NMR spectroscopy of membrane proteins, we have implemented several different (13)C labeling schemes with the purpose of overcoming the interfering effects of (13)C-(13)C dipole-dipole couplings in stationary samples. The membrane-bound form of the major coat protein of the filamentous bacteriophage Pf1 was used as an example of a well-characterized helical membrane protein. Aligned protein samples randomly enriched to 35% (13)C in all sites and metabolically labeled from bacterial growth on media containing [2-(13)C]-glycerol or [1,3-(13)C]-glycerol enables direct (13)C detection in solid-state NMR experiments without the need for homonuclear (13)C-(13)C dipole-dipole decoupling. The (13)C-detected NMR spectra of Pf1 coat protein show a substantial increase in sensitivity compared to the equivalent (15)N-detected spectra. The isotopic labeling pattern was analyzed for [2-(13)C]-glycerol and [1,3-(13)C]-glycerol as metabolic precursors by solution-state NMR of micelle samples. Polarization inversion spin exchange at the magic angle (PISEMA) and other solid-state NMR experiments work well on 35% random fractionally and metabolically tailored (13)C-labeled samples, in contrast to their failure with conventional 100% uniformly (13)C-labeled samples
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, N.I.H., Extramural
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a Carbon Isotopes
|2 NLM
|
| 650 |
|
7 |
|a Membrane Proteins
|2 NLM
|
| 650 |
|
7 |
|a Viral Core Proteins
|2 NLM
|
| 650 |
|
7 |
|a Viral Structural Proteins
|2 NLM
|
| 700 |
1 |
|
|a Filipp, Fabian V
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Jairam, Lena
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Park, Sang Ho
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Bradley, Joel
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Opella, Stanley J
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Magnetic resonance in chemistry : MRC
|d 1985
|g 45 Suppl 1(2007) vom: 25. Dez., Seite S107-15
|w (DE-627)NLM098179667
|x 1097-458X
|7 nnns
|
| 773 |
1 |
8 |
|g volume:45 Suppl 1
|g year:2007
|g day:25
|g month:12
|g pages:S107-15
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 45 Suppl 1
|j 2007
|b 25
|c 12
|h S107-15
|