Polymeric brushes as functional templates for immobilizing ribonuclease A : study of binding kinetics and activity

The ability to immobilize proteins with high binding capacities on surfaces while maintaining their activity is critical for protein microarrays and other biotechnological applications. We employed poly(acrylic acid) (PAA) brushes as templates to immobilize ribonuclease A (RNase A), which is commonl...

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Détails bibliographiques
Publié dans:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 24(2008), 3 vom: 05. Feb., Seite 913-20
Auteur principal: Cullen, Sean P (Auteur)
Autres auteurs: Liu, Xiaosong, Mandel, Ian C, Himpsel, Franz J, Gopalan, Padma
Format: Article
Langue:English
Publié: 2008
Accès à la collection:Langmuir : the ACS journal of surfaces and colloids
Sujets:Journal Article Research Support, U.S. Gov't, Non-P.H.S. Acrylic Resins Coated Materials, Biocompatible Enzymes, Immobilized carbopol 940 4Q93RCW27E Ribonuclease, Pancreatic EC 3.1.27.5 Silicon Z4152N8IUI