Polymeric brushes as functional templates for immobilizing ribonuclease A : study of binding kinetics and activity

The ability to immobilize proteins with high binding capacities on surfaces while maintaining their activity is critical for protein microarrays and other biotechnological applications. We employed poly(acrylic acid) (PAA) brushes as templates to immobilize ribonuclease A (RNase A), which is commonl...

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Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 24(2008), 3 vom: 05. Feb., Seite 913-20
1. Verfasser: Cullen, Sean P (VerfasserIn)
Weitere Verfasser: Liu, Xiaosong, Mandel, Ian C, Himpsel, Franz J, Gopalan, Padma
Format: Aufsatz
Sprache:English
Veröffentlicht: 2008
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, U.S. Gov't, Non-P.H.S. Acrylic Resins Coated Materials, Biocompatible Enzymes, Immobilized carbopol 940 4Q93RCW27E Ribonuclease, Pancreatic EC 3.1.27.5 Silicon Z4152N8IUI