Continuous enzymatic assay for histone lysine methyltransferases
We describe a continuous peptide methylation assay using the Neurospora crassa Dim-5 histone H3 lysine 9 (H3K9) methyltransferase as a model system. The assay uses streptavidin FlashPlates coated with target peptide. Since no washing and pipeting steps were required after the addition of the enzyme/...
| Publié dans: | BioTechniques. - 1991. - 43(2007), 5 vom: 01. Nov., Seite 602, 604, 606 passim |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
2007
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Peptides S-Adenosylmethionine 7LP2MPO46S Histone-Lysine N-Methyltransferase EC 2.1.1.43 |