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|a eng
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| 100 |
1 |
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|a Spijkerman, Elly
|e verfasserin
|4 aut
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|a Phosphorus acquisition by Chlamydomonas acidophila under autotrophic and osmo-mixotrophic growth conditions
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|c 2007
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 28.04.2008
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|a Date Revised 24.11.2016
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Chlamydomonas acidophila Negoro is a green algal species abundant in acidic waters where inorganic phosphorus (P(i)) and carbon (CO(2)) are considered the most important growth-limiting nutrients for the phytoplankton. This paper describes the P(i) uptake and growth kinetics under varying carbon supply by cultivating the alga autotrophically, with and without CO(2) aeration, and osmo-mixotrophically with glucose under low P(i) conditions at pH 2.7. The low minimum cellular phosphorus quota (Q(0); ranging from 0.6 to 1.1 mmol P mol(-1) C) suggested P(i)-limiting conditions under all different modes of carbon supply, and was lowest under CO(2)-aerated conditions. The threshold P(i) concentration for growth did not vary from zero, suggesting no detectable metabolic costs. Maximum P(i)-uptake rates (V(max)) were a better indication of P(i) limitation when compared with the affinity constant for P(i) uptake (K(m)), as V(max) was only high under P(i)-limited conditions whereas K(m) was low under both P(i)-limited and P(i)-replete conditions. Osmo-mixotrophic growth conditions did not result in decreased extracellular phosphatase activity, but often resulted in physiological characteristics comparable with CO(2)-aerated cells, suggesting intracellular CO(2) production by glucose respiration. In addition, at low CO(2) and in autotrophic conditions, C. acidophila had a higher Q(0), lower dissolved organic carbon concentration, lower maximum P(i)-uptake rates, and lower phosphatase activity, suggesting that growth was co-limited by CO(2) and P(i). Furthermore, cells may respond physiologically to both nutrient limitations simultaneously
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Carbon Dioxide
|2 NLM
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|a 142M471B3J
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|a Phosphorus
|2 NLM
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|2 NLM
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|a Phosphoric Monoester Hydrolases
|2 NLM
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| 650 |
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|a EC 3.1.3.2
|2 NLM
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| 650 |
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|a Glucose
|2 NLM
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| 650 |
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 58(2007), 15-16 vom: 25., Seite 4195-202
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|x 1460-2431
|7 nnas
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|g year:2007
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|g day:25
|g pages:4195-202
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