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231223s2007 xx |||||o 00| ||eng c |
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|a 10.1111/j.1469-8137.2007.02214.x
|2 doi
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|a pubmed24n0582.xml
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|a (NLM)17953540
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wu, Yansheng
|e verfasserin
|4 aut
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|a Heterotrimeric G-protein participation in Arabidopsis pollen germination through modulation of a plasmamembrane hyperpolarization-activated Ca2+-permeable channel
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|c 2007
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 12.02.2008
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|a Date Revised 14.04.2021
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|a published: Print
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|a Citation Status MEDLINE
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|a The role of heterotrimeric G proteins in pollen germination and tube growth was investigated using Arabidopsis thaliana plants in which the gene (GPA) encoding the G-protein a subunit (Galpha) was null or overexpressed. Pollen germination, free cytosolic calcium concentration ([Ca(2+)](cyt)) and Ca(2+) channel activity in the plasma membrane (PM) of pollen cells were investigated. Results showed that, compared with pollen grains of the wild type (ecotype Wassilewskija, ws), in vitro germinated pollen of Galpha null mutants (gpa1-1 and gpa1-2) had lower germination percentages and shorter pollen tubes, while pollen from Galpha overexpression lines (wGalpha and cGalpha) had higher germination percentages and longer pollen tubes. Compared with ws pollen cells, [Ca(2+)](cyt) was lower in gpa1-1 and gpa1-2 and higher in wGalpha and cGalpha. In whole-cell patch clamp recordings, a hyperpolarization-activated Ca(2+)-permeable conductance was identified in the PM of pollen protoplasts. The conductance was suppressed by trivalent cations but insensitive to organic blockers; its permeability to divalent cations was Ba(2+) > Ca(2+) > Mg(2+) > Sr(2+) > Mn(2+). The activity of the Ca(2+)-permeable channel conductance was down-regulated in pollen protoplasts of gpa1-1 and gpa1-2, and up-regulated in wGalpha and cGalpha. The results suggest that Galpha may participate in pollen germination through modulation of the hyperpolarization-activated Ca(2+) channel in the PM of pollen cells
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis Proteins
|2 NLM
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|a Calcium Channels
|2 NLM
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|a GPA1 protein, Arabidopsis
|2 NLM
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| 650 |
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|a GTP-Binding Protein alpha Subunits
|2 NLM
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| 650 |
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|a Calcium
|2 NLM
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| 650 |
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|a SY7Q814VUP
|2 NLM
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| 700 |
1 |
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|a Xu, Xiaodong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Sujuan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Ting
|e verfasserin
|4 aut
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| 700 |
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|a Ma, Ligeng
|e verfasserin
|4 aut
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| 700 |
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|a Shang, Zhonglin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 176(2007), 3 vom: 15., Seite 550-559
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:176
|g year:2007
|g number:3
|g day:15
|g pages:550-559
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|u http://dx.doi.org/10.1111/j.1469-8137.2007.02214.x
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