Oriented purple membrane monolayers covalently attached to gold by multiple thiole linkages analyzed by single molecule force spectroscopy
Highly oriented monolayers of bacteriorhodopsin (BR) in purple membrane (PM) form are obtained by the reaction of BR-Q3C, where a cysteine was introduced into the N-terminal region, with a gold surface. Single molecule force spectroscopy was used to show that about 50% of the BRs are covalently boun...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1991. - 23(2007), 22 vom: 23. Okt., Seite 11134-8 |
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| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2007
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Coated Materials, Biocompatible Membranes, Artificial Sulfhydryl Compounds Bacteriorhodopsins 53026-44-1 Gold 7440-57-5 |
| Zusammenfassung: | Highly oriented monolayers of bacteriorhodopsin (BR) in purple membrane (PM) form are obtained by the reaction of BR-Q3C, where a cysteine was introduced into the N-terminal region, with a gold surface. Single molecule force spectroscopy was used to show that about 50% of the BRs are covalently bound to the surface. The linkage between the cysteine and the gold causes an additional characteristic peak in the force-distance curves to appear. Because several thousand cysteine-gold bonds exist between each PM patch and the surface, the PM is irreversibly bound. Such oriented PM monolayers may serve as an interface between metal surfaces and biomaterials, which may be linked to the PM surface chemically. Photoelectric applications of BR will benefit from the high degree of orientation obtained by this method |
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| Beschreibung: | Date Completed 20.12.2007 Date Revised 16.10.2007 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 0743-7463 |