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01000caa a22002652 4500 |
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NLM173357024 |
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DE-627 |
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20241127232013.0 |
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tu |
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231223s2007 xx ||||| 00| ||eng c |
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|a pubmed24n1614.xml
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|a (DE-627)NLM173357024
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|a (NLM)17825626
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Zhang, Li
|e verfasserin
|4 aut
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| 245 |
1 |
2 |
|a A robust immunoassay for anti-interferon autoantibodies that is highly specific for patients with autoimmune polyglandular syndrome type 1
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| 264 |
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1 |
|c 2007
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 18.12.2007
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| 500 |
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|a Date Revised 27.11.2024
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| 500 |
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a UNLABELLED: High titer antibodies to type 1 interferons have been recently reported as being highly specific for patients with autoimmune polyglandular syndrome type 1 (APS1) in Finnish and Norwegian patients with mutations in the AIRE gene. Those studies employed a complex neutralization assay to define the type 1 interferon autoantibodies. Here we have established a competitive europium time resolved fluorescence assay for IFN-alpha autoantibodies and measured sera from subjects with APS1, first degree relatives of APS1 patients, patients with Addison's disease or Type 1 diabetes. The europium-based immunoassay utilizes plate bound human IFN-alpha incubated with sera with or without competition with fluid phase IFN-alpha, followed by anti-IgG biotinylated antibody and detection with streptavidin-europium. The index of IFN-alpha Ab was calculated as (CPS (Counts per second) without competition-CPS with competition)/(CPS positive standard sera without competition-CPS positive standard sera with competition). RESULTS are reported for raw CPS and indices and are compared across the different subjects
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| 520 |
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|a RESULTS: For normal controls (n=100) CPS without competition were 31,237+/-17,328 CPS while after subtracting the competition value, the results were -6563+/-10,303 CPS. The initial APS1 patient (used to create the index as 1.0) gave 394,063 CPS without competition and a delta of 363,662+/-31,587 CPS with competition. Scatchard plot analysis of this patient sample revealed a high avidity for IFN-alpha (K(d) of 0.5 nM). The CPS, delta, and index for 6/7 APS1 patients were strongly positive and 3 standard deviations or more above that of the normal controls. Using a cut-off of 2 standard deviations above normal controls, relatives of APS1 patients were negative for type I interferon autoantibodies as were 71 patients with Addison's disease (non-APS1) and 141 Type 1 diabetes patients. This simple high throughput competitive europium time resolved fluorescence assay had a sensitivity of > or =86% or greater and a specificity of >99.5%
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| 650 |
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|a Comparative Study
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, N.I.H., Extramural
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Autoantibodies
|2 NLM
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| 650 |
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7 |
|a Interferon-alpha
|2 NLM
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| 650 |
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7 |
|a Luminescent Agents
|2 NLM
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| 650 |
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7 |
|a Europium
|2 NLM
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| 650 |
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7 |
|a 444W947O8O
|2 NLM
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| 700 |
1 |
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|a Barker, Jennifer M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Babu, Sunanda
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Su, Maureen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Stenerson, Matthew
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cheng, Mickie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shum, Anthony
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zamir, Ehud
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Badolato, Raffaele
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Law, Adam
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Eisenbarth, George S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Anderson, Mark S
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 125(2007), 2 vom: 18. Nov., Seite 131-7
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
1 |
8 |
|g volume:125
|g year:2007
|g number:2
|g day:18
|g month:11
|g pages:131-7
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_11
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| 912 |
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|a GBV_ILN_24
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 125
|j 2007
|e 2
|b 18
|c 11
|h 131-7
|