Decline of arbuscular mycorrhizal fungi in northern hardwood forests exposed to chronic nitrogen additions

Arbuscular mycorrhizal (AM) fungi are important below-ground carbon (C) sinks that can be sensitive to increased nitrogen (N) availability. The abundance of AM fungi (AMF) was estimated in maple (Acer spp.) fine roots following more than a decade of experimental additions of N designed to simulate c...

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Veröffentlicht in:The New phytologist. - 1990. - 176(2007), 1 vom: 01., Seite 175-183
1. Verfasser: Van Diepen, Linda T A (VerfasserIn)
Weitere Verfasser: Lilleskov, Erik A, Pregitzer, Kurt S, Miller, R Michael
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, U.S. Gov't, Non-P.H.S. Carbon 7440-44-0 Nitrogen N762921K75
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520 |a Arbuscular mycorrhizal (AM) fungi are important below-ground carbon (C) sinks that can be sensitive to increased nitrogen (N) availability. The abundance of AM fungi (AMF) was estimated in maple (Acer spp.) fine roots following more than a decade of experimental additions of N designed to simulate chronic atmospheric N deposition. Abundance of AMF was measured by staining and ocular estimation, as well as by analyzing for the AMF indicator fatty acid 16:1omega5c in phospholipid (biomass indicator) and neutral lipid (lipid storage indicator) fractions. Arbuscular mycorrhizal fungal biomass, storage structures and lipid storage declined in response to N addition measured by both methods. This pattern was found when AM response was characterized as colonization intensity, on an areal basis and in proportion to maple above-ground biomass. The phospholipid fraction of the fatty acid 16:1omega5c was positively correlated with total AMF colonization and the neutral lipid fraction with vesicle colonization. Decreased AMF abundance with simulated N deposition suggests reduced C allocation to these fungi or a direct soil N-mediated decline. The fatty acid (phospholipid and neutral lipid fractions) 16:1omega5c was found to be a good indicator for AMF active biomass and stored energy, respectively 
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700 1 |a Lilleskov, Erik A  |e verfasserin  |4 aut 
700 1 |a Pregitzer, Kurt S  |e verfasserin  |4 aut 
700 1 |a Miller, R Michael  |e verfasserin  |4 aut 
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