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20250208143449.0 |
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231223s2007 xx ||||| 00| ||eng c |
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|a pubmed25n0574.xml
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|a (DE-627)NLM172308909
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|a (NLM)17718710
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Knüsel, R
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Virus isolation vs RT-PCR
|b which method is more successful in detecting VHSV and IHNV in fish tissue sampled under field conditions?
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|c 2007
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 19.10.2007
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a This study compared the results of reverse transcription-polymerase chain reaction (RT-PCR) and traditional virus isolation on cell culture in detection of viral haemorrhagic septicaemia virus (VHSV) and infectious haematopoietic necrosis virus (IHNV). RT-PCR was used for 172 tissue sample pools (total of 859 fish) originating from a field survey on the occurrence of VHSV and IHNV in farmed and wild salmonids in Switzerland. These samples represented all sites with fish that were either identified as virus-positive by means of virus isolation (three sites, four positive tissue sample pools) and/or demonstrated positive anti-VHSV-antibody titres (83 sites, 121 positive blood samples) in a serum plaque neutralization test (SPNT). The RT-PCR technique confirmed the four VHSV-positive tissue sample pools detected by virus isolation and additionally identified one VHSV-positive sample that showed positive anti-VHSV-AB titres, but was negative in virus isolation. With IHNV, RT-PCR detected two positive samples not identified by virus isolation while in these fish the SPNT result had been questionable. One of the IHNV-positive samples represents the first detection of IHNV-RNA in wild brown trout in Switzerland. Compared to SPNT, the RT-PCR method detected, as with virus isolation, a much lower number of positive cases; reasons for this discrepancy are discussed. Our results indicate that RT-PCR can not only be successfully applied in field surveys, but may also be slightly more sensitive than virus isolation. However, in a titration experiment under laboratory conditions, the sensitivity of RT-PCR was not significantly higher when compared with virus isolation
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| 650 |
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|a Evaluation Study
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 700 |
1 |
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|a Bergmann, S M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Einer-Jensen, K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Casey, J
|e verfasserin
|4 aut
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| 700 |
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|a Segner, H
|e verfasserin
|4 aut
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| 700 |
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|a Wahli, T
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 30(2007), 9 vom: 21. Sept., Seite 559-68
|w (DE-627)NLM098166034
|x 0140-7775
|7 nnns
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| 773 |
1 |
8 |
|g volume:30
|g year:2007
|g number:9
|g day:21
|g month:09
|g pages:559-68
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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| 952 |
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|d 30
|j 2007
|e 9
|b 21
|c 09
|h 559-68
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