High-throughput profiling of posttranslational modification enzymes by phage display
Phage display has been used as a high-throughput platform for identifying proteins or peptides with desired binding or catalytic activities from a complex proteome. Recently, phage display has been applied to profile the catalytic activities of posttranslational modification (PTM) enzymes. Here, we...
| Veröffentlicht in: | BioTechniques. - 1993. - 43(2007), 1 vom: 15. Juli, Seite 31, 33, 35 passim |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2007
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Acyl Carrier Protein Bacterial Proteins Enzymes Peptide Library phosphopantetheinyl transferase Pantetheine 496-65-1 Polyketide Synthases mehr... |
| Zusammenfassung: | Phage display has been used as a high-throughput platform for identifying proteins or peptides with desired binding or catalytic activities from a complex proteome. Recently, phage display has been applied to profile the catalytic activities of posttranslational modification (PTM) enzymes. Here, we highlight recent work elucidating the downstream targets of PTM enzymes by phage display, including the genome-wide profiling of biosynthetic enzymes subject to phosphopantetheinyl transferase (PPTase) modification |
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| Beschreibung: | Date Completed 30.08.2007 Date Revised 07.06.2018 published: Print Citation Status MEDLINE |
| ISSN: | 0736-6205 |