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231223s2007 xx ||||| 00| ||eng c |
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|a pubmed24n0571.xml
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|a (NLM)17616154
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Nabok, Alexei
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Specific binding of large aggregates of amphiphilic molecules to the respective antibodies
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| 264 |
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|c 2007
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 02.10.2007
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|a Date Revised 24.11.2016
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The Binding of nonylphenol to respective antibodies immobilized on solid substrates was studied with the methods of total internal reflection ellipsometry (TIRE) and QCM (quartz crystal microbalance) impedance spectroscopy. The binding reaction was proved to be highly specific having an association constant of KA=1.6x10(6) mol(-1) L and resulted in an increase in both the adsorbed layer thickness of 23 nm and the added mass of 18.3 microg/cm2 at saturation. The obtained responses of both TIRE and QCM methods are substantially higher than anticipated for the immune binding of single molecules of nonylphenol. The mechanism of binding of large aggregates of nonylphenol was suggested instead. Modeling of the micelle of amphiphilic nonylphenol molecules in aqueous solutions yielded a micelle size of about 38 nm. The mechanism of binding of large molecular aggregates to respective antibodies can be extended to other hydrophobic low-molecular-weight toxins such as T-2 mycotoxin. The formation of large molecular aggregates of nonylphenol and T-2 mycotoxin molecules on the surface was proved by the AFM study
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| 650 |
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Antibodies
|2 NLM
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|a Micelles
|2 NLM
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| 650 |
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|a Phenols
|2 NLM
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| 650 |
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|a nonylphenol
|2 NLM
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| 650 |
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7 |
|a 79F6A2ILP5
|2 NLM
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| 650 |
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|a T-2 Toxin
|2 NLM
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| 650 |
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7 |
|a I3FL5NM3MO
|2 NLM
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| 700 |
1 |
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|a Tsargorodskaya, Anna
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Holloway, Alan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Starodub, Nikolay F
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Demchenko, Anna
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 23(2007), 16 vom: 31. Juli, Seite 8485-90
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:23
|g year:2007
|g number:16
|g day:31
|g month:07
|g pages:8485-90
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