Influence of salt on colloidal lithography of albumin

We investigate the influence of salt on colloidal lithography of biomolecular patterns. Albumin labeled with fluorescein isothiocyanate (FITC) was adsorbed on polyelectrolyte-coated glass substrates covered by negatively charged colloids using fluorescence microscopy. After removing the colloids, a...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1991. - 23(2007), 16 vom: 31. Juli, Seite 8480-4
1. Verfasser: Geng, D L (VerfasserIn)
Weitere Verfasser: Miao, Y H, Helseth, L E
Format: Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Albumins Colloids Salts Fluorescein-5-isothiocyanate I223NX31W9
Beschreibung
Zusammenfassung:We investigate the influence of salt on colloidal lithography of biomolecular patterns. Albumin labeled with fluorescein isothiocyanate (FITC) was adsorbed on polyelectrolyte-coated glass substrates covered by negatively charged colloids using fluorescence microscopy. After removing the colloids, a well-defined albumin pattern remains, and we study how the pattern changes upon adding salt to the protein solution. The proposed method is simple and cheap and can be used to create stable one- and two-dimensional biomolecular arrays
Beschreibung:Date Completed 02.10.2007
Date Revised 21.11.2013
published: Print-Electronic
Citation Status MEDLINE
ISSN:0743-7463