The 9-lipoxygenase GhLOX1 gene is associated with the hypersensitive reaction of cotton Gossypium hirsutum to Xanthomonas campestris pv malvacearum

Hypersensitive reaction (HR) cell death of cotton to the incompatible race 18 from Xanthomonas campestris pathovar malvacearum (Xcm) is associated with 9S-lipoxygenase activity (LOX) responsible for lipid peroxidation. Here, we report the cloning of cotton (Gossypium hirsutum L.) LOX gene (GhLOX1) a...

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Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 45(2007), 8 vom: 01. Aug., Seite 596-606
1. Verfasser: Marmey, Philippe (VerfasserIn)
Weitere Verfasser: Jalloul, Aïda, Alhamdia, Majd, Assigbetse, Komi, Cacas, Jean-Luc, Voloudakis, Andreas E, Champion, Antony, Clerivet, Alain, Montillet, Jean-Luc, Nicole, Michel
Format: Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Acetates Cyclopentanes Oxylipins methyl jasmonate 900N171A0F Hydrogen Peroxide BBX060AN9V Lipoxygenase EC 1.13.11.12 mehr... Salicylic Acid O414PZ4LPZ
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245 1 4 |a The 9-lipoxygenase GhLOX1 gene is associated with the hypersensitive reaction of cotton Gossypium hirsutum to Xanthomonas campestris pv malvacearum 
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520 |a Hypersensitive reaction (HR) cell death of cotton to the incompatible race 18 from Xanthomonas campestris pathovar malvacearum (Xcm) is associated with 9S-lipoxygenase activity (LOX) responsible for lipid peroxidation. Here, we report the cloning of cotton (Gossypium hirsutum L.) LOX gene (GhLOX1) and the sequencing of its promoter. GhLOX1 was found to be highly expressed during Xcm induced HR. Sequence analysis showed that GhLOX1 is a putative 9-LOX, and GhLOX1 promoter contains SA and JA responsive elements. Investigation on LOX signalisation on cotyledons infiltrated with salicylic acid (SA), or incubated with methyl-jasmonate (MeJA) revealed that both treatments induced LOX activity and GhLOX1 gene expression. HR-like symptoms were observed when LOX substrates were then injected in treated (MeJA and SA) cotyledons or when Xcm compatible race 20 was inoculated on MeJA treated cotyledons. Together these results support the fact that GhLOX1 encodes a 9 LOX whose activity would be involved in cell death during cotton HR 
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650 7 |a Salicylic Acid  |2 NLM 
650 7 |a O414PZ4LPZ  |2 NLM 
700 1 |a Jalloul, Aïda  |e verfasserin  |4 aut 
700 1 |a Alhamdia, Majd  |e verfasserin  |4 aut 
700 1 |a Assigbetse, Komi  |e verfasserin  |4 aut 
700 1 |a Cacas, Jean-Luc  |e verfasserin  |4 aut 
700 1 |a Voloudakis, Andreas E  |e verfasserin  |4 aut 
700 1 |a Champion, Antony  |e verfasserin  |4 aut 
700 1 |a Clerivet, Alain  |e verfasserin  |4 aut 
700 1 |a Montillet, Jean-Luc  |e verfasserin  |4 aut 
700 1 |a Nicole, Michel  |e verfasserin  |4 aut 
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