Identification of B-cell epitopes on the betanodavirus capsid protein
The pepscan procedure was used to identify betanodavirus B-cell epitopes recognized by neutralizing mouse monoclonal antibodies (MAbs) and serum samples obtained from sea bass, Dicentrarchus labrax, naturally infected with betanodavirus. Pepscan was performed with a panel of thirty-four 12-mer synth...
| Publié dans: | Journal of fish diseases. - 1998. - 30(2007), 7 vom: 15. Juli, Seite 419-26 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2007
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| Accès à la collection: | Journal of fish diseases |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Antibodies, Monoclonal Capsid Proteins Epitopes, B-Lymphocyte |
| Résumé: | The pepscan procedure was used to identify betanodavirus B-cell epitopes recognized by neutralizing mouse monoclonal antibodies (MAbs) and serum samples obtained from sea bass, Dicentrarchus labrax, naturally infected with betanodavirus. Pepscan was performed with a panel of thirty-four 12-mer synthetic peptides that mimicked the entire betanodavirus capsid protein. Sea bass serum samples reacted strongly with three regions of the capsid protein comprising amino acid residues 1-32, 91-162 and 181-212. The latter region was also recognized by neutralizing MAbs and coincided with a region of high antigenic propensity identified by an antigen prediction algorithm. These data suggest that a region of the betanodavirus capsid protein spanning amino acid residues 181-212 may represent a neutralization domain that could potentially be used to inform the development of nodavirus vaccines and immunodiagnostic reagents |
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| Description: | Date Completed 28.09.2007 Date Revised 30.09.2020 published: Print Citation Status MEDLINE |
| ISSN: | 1365-2761 |