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231223s2007 xx ||||| 00| ||eng c |
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Chopin, Franck
|e verfasserin
|4 aut
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| 245 |
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|a The Arabidopsis nitrate transporter AtNRT2.1 is targeted to the root plasma membrane
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|c 2007
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 03.12.2007
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Arabidopsis AtNRT2.1 protein is the best characterized high affinity nitrate transporter in higher plants. However, nothing is known about its sub-cellular localization. In this work, we used GFP imaging to follow the targeting of the AtNRT2.1 protein to the different cell membranes. A polyclonal antibody was also raised against a peptide derived from the AtNRT2.1 sequence. Comparison of wild type and mutant plant extracts showed that this antibody recognized specifically the AtNRT2.1 protein. Microsomal membranes were fractionated on sucrose gradients and immunological detections were performed on the different fractions. Altogether, our results demonstrate that the AtNRT2.1 protein is located in the plasma membrane of the root cells
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|a Journal Article
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|a Anion Transport Proteins
|2 NLM
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| 650 |
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|a Arabidopsis Proteins
|2 NLM
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|a AtNRT2.1 protein, Arabidopsis
|2 NLM
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| 650 |
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|a Nitrates
|2 NLM
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| 650 |
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|a Green Fluorescent Proteins
|2 NLM
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| 650 |
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|a 147336-22-9
|2 NLM
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| 700 |
1 |
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|a Wirth, Judith
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dorbe, Marie-France
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lejay, Laurence
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Krapp, Anne
|e verfasserin
|4 aut
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| 700 |
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|a Gojon, Alain
|e verfasserin
|4 aut
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| 700 |
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|a Daniel-Vedele, Françoise
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 45(2007), 8 vom: 01. Aug., Seite 630-5
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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8 |
|g volume:45
|g year:2007
|g number:8
|g day:01
|g month:08
|g pages:630-5
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