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NLM170987736 |
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DE-627 |
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20231223124714.0 |
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231223s2007 xx ||||| 00| ||eng c |
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|a pubmed24n0570.xml
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|a (DE-627)NLM170987736
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|a (NLM)17579466
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Seker, Urartu Ozgur Safak
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Adsorption behavior of linear and cyclic genetically engineered platinum binding peptides
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| 264 |
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1 |
|c 2007
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 24.10.2007
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a Recently, phage and cell-surface display libraries have been adapted for genetically selecting short peptides for a variety of inorganic materials. Despite the enormous number of inorganic-binding peptides reported and their bionanotechnological utility as synthesizers and molecular linkers, there is still a limited understanding of molecular mechanisms of peptide recognition of and binding to solid materials. As part of our goal of genetically designing these peptides, understanding the binding kinetics and thermodynamics, and using the peptides as molecular erectors, in this report we discuss molecular structural constraints imposed upon the quantitative binding characteristics of peptides with an affinity for inorganics. Specifically, we use a high-affinity seven amino acid Pt-binding sequence, PTSTGQA, as we reported in earlier studies and build two constructs: one is a Cys-Cys constrained "loop" sequence (CPTSTGQAC) that mimics the domain used in the pIII tail sequence of the phage library construction, and the second is the linear form, a septapeptide, without the loop. Both sequences were analyzed for their adsorption behavior on Pt thin films by surface plasmon resonance (SPR) spectroscopy and for their conformational properties by circular dichroism (CD). We find that the cyclic peptide of the integral Pt-binding sequence possesses single or 1:1 Langmuir adsorption behavior and displays equilibrium and adsorption rate constants that are significantly larger than those obtained for the linear form. Conversely, the linear form exhibits biexponential Langmuir isotherm behavior with slower and weaker binding. Furthermore, the structure of the cyclic version was found to adopt a random coil molecular conformation, whereas the linear version adopts a polyproline type II conformation in equilibrium with the random coil. The 2,2,2-trifluoroethanol titration experiments indicate that TFE has a different effect on the secondary structures of the linear and cyclic versions of the Pt binding sequence. We conclude that the presence of the Cys-Cys restraint affects both the conformation and binding behavior of the integral Pt-binding septapeptide sequence and that the presence or absence of constraints could be used to tune the adsorption and structural features of inorganic binding peptide sequences
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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7 |
|a Peptide Library
|2 NLM
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| 650 |
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7 |
|a Peptides
|2 NLM
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| 650 |
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|a Peptides, Cyclic
|2 NLM
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| 650 |
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7 |
|a polyproline
|2 NLM
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| 650 |
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7 |
|a 25191-13-3
|2 NLM
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| 650 |
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7 |
|a Platinum
|2 NLM
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| 650 |
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7 |
|a 49DFR088MY
|2 NLM
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| 700 |
1 |
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|a Wilson, Brandon
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dincer, Sevil
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kim, Il Won
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Oren, Ersin Emre
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Evans, John Spencer
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tamerler, Candan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sarikaya, Mehmet
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 23(2007), 15 vom: 17. Juli, Seite 7895-900
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
8 |
|g volume:23
|g year:2007
|g number:15
|g day:17
|g month:07
|g pages:7895-900
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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|a GBV_ILN_22
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|a GBV_ILN_350
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| 912 |
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|a GBV_ILN_721
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| 951 |
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|a AR
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| 952 |
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|d 23
|j 2007
|e 15
|b 17
|c 07
|h 7895-900
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