Enhanced salt tolerance of transgenic progeny of tall fescue (Festuca arundinacea) expressing a vacuolar Na+/H+ antiporter gene from Arabidopsis
Salinity is a major abiotic stress factor limiting crop production. To generate salt-tolerant turf and forage, we had transformed tall fescue (Festuca arundinacea) with AtNHX1, a vacuolar Na(+)/H(+) antiporter gene from Arabidopsis thaliana. In this paper, we report that overexpression of the AtNHX1...
| Veröffentlicht in: | Journal of plant physiology. - 1979. - 164(2007), 10 vom: 01. Okt., Seite 1377-83 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2007
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| Zugriff auf das übergeordnete Werk: | Journal of plant physiology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Sodium-Hydrogen Exchangers Sodium Chloride 451W47IQ8X Sodium 9NEZ333N27 Potassium RWP5GA015D |
| Zusammenfassung: | Salinity is a major abiotic stress factor limiting crop production. To generate salt-tolerant turf and forage, we had transformed tall fescue (Festuca arundinacea) with AtNHX1, a vacuolar Na(+)/H(+) antiporter gene from Arabidopsis thaliana. In this paper, we report that overexpression of the AtNHX1 gene confers enhanced salt tolerance to the transformed tall fescue progenies. DNA gel blot analysis and reverse transcription (RT) polymerase chain reaction (PCR) were carried out to confirm the inheritance and expression of the AtNHX1 gene in transgenic T(1) and T(2) lines. These transgenic lines showed no phenotypic changes or yield reduction. Plants carrying the AtNHX1 gene were more resistant to a 20 mM NaCl solution than control plants. The roots of the transgenic lines had a higher sodium content than controls, due to an increased Na(+)/H(+) antiporter activity in tonoplast vesicles. Our results suggest that this accumulation of sodium in vacuoles of root cells, mediated by vacuolar Na(+)/H(+) antiporters, reduced the toxic effects of salinity to tall fescue and thus enhanced its salt tolerance |
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| Beschreibung: | Date Completed 04.01.2008 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1618-1328 |