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231223s2007 xx ||||| 00| ||eng c |
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|a pubmed24n0569.xml
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|a (DE-627)NLM170759237
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|a (NLM)17555338
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wang, Chengshan
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Surface properties of "jellyfish"
|b Langmuir monolayer and Langmuir-Blodgett film studies of recombinant aequorin
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|c 2007
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 14.09.2007
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a In this paper, we studied the surface properties of recombinant aequorin at the air-water interface. Using the Langmuir monolayer technique, the surface properties of aequorin were studied, including the surface pressure and surface potential-area isotherms, compression-decompression cycles, and stability on Trizma Base (Tris/HCl) buffer at pH 7.6. The results showed that aequorin formed a stable Langmuir monolayer and the surface pressure-area isotherms were dependent on both pH and ionic strength. At a pH higher or lower than 7.6, the limiting molecular area decreased. The circular dichroism (CD) spectra of aequorin in aqueous solutions explained this result: when the pH was higher than 7.6, the alpha-helix conformation changed to unordered structures, whereas at a pH lower than 7.6, the alpha-helix conformation changed to beta-sheet. The addition of calcium chloride to the Tris/HCl buffer subphase (pH 7.6) caused an increase of the limiting molecular area of the aequorin Langmuir monolayer. The fluorescence spectra of a Langmuir-Blodgett (LB) film of aequorin in the presence of calcium chloride indicated that the aequorin transformed to the apoaequorin
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Apoproteins
|2 NLM
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|a Buffers
|2 NLM
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|a Luminescent Agents
|2 NLM
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|a Recombinant Proteins
|2 NLM
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|a apoaequorin
|2 NLM
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|a Water
|2 NLM
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| 650 |
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|a 059QF0KO0R
|2 NLM
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|a Aequorin
|2 NLM
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|a 50934-79-7
|2 NLM
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| 650 |
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|a Potassium Chloride
|2 NLM
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| 650 |
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|a 660YQ98I10
|2 NLM
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|a Calcium Chloride
|2 NLM
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| 650 |
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|a M4I0D6VV5M
|2 NLM
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| 700 |
1 |
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|a Micic, Miodrag
|e verfasserin
|4 aut
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1 |
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|a Ensor, Mark
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Daunert, Sylvia
|e verfasserin
|4 aut
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| 700 |
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|a Leblanc, Roger M
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 23(2007), 14 vom: 03. Juli, Seite 7602-7
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:23
|g year:2007
|g number:14
|g day:03
|g month:07
|g pages:7602-7
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|a GBV_USEFLAG_A
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|a AR
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|d 23
|j 2007
|e 14
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|c 07
|h 7602-7
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