Adsorption of bovine serum albumin on template-polymerized chitosan/poly(methacrylic acid) complexes

Particulate systems composed of polyelectrolyte complexes (PEC) based on chitosan and poly(methacrylic acid) were obtained via template polymerization. The resultant particles were characterized as having regions with different charge densities: chitosan predominating in the core and poly(methacryli...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 23(2007), 14 vom: 03. Juli, Seite 7687-94
1. Verfasser: de Vasconcelos, C L (VerfasserIn)
Weitere Verfasser: Bezerril, P M, Dantas, T N C, Pereira, M R, Fonseca, J L C
Format: Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Electrolytes Polymers Polymethacrylic Acids Serum Albumin, Bovine 27432CM55Q Chitosan 9012-76-4 Glutaral T3C89M417N
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245 1 0 |a Adsorption of bovine serum albumin on template-polymerized chitosan/poly(methacrylic acid) complexes 
264 1 |c 2007 
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520 |a Particulate systems composed of polyelectrolyte complexes (PEC) based on chitosan and poly(methacrylic acid) were obtained via template polymerization. The resultant particles were characterized as having regions with different charge densities: chitosan predominating in the core and poly(methacrylic acid) at the surface, the particles being negatively charged, as a consequence. Albumin was adsorbed on these particles (after cross-linking with glutardialdehyde), and pH was controlled to obtain two conditions: (i) adsorption of positively charged albumin and (ii) adsorption of albumin at its isoelectric point. Adsorption isotherms and zeta-potential measurements showed that albumin adsorption was controlled by hydrogen bonding/van der Waals interactions and that brush-like structures may enhance the adsorption of albumin on these particles. It was also found that shearing can induce desorption of albumin from the PEC surface, depending on the continuous phase albumin concentration 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Polymers  |2 NLM 
650 7 |a Polymethacrylic Acids  |2 NLM 
650 7 |a Serum Albumin, Bovine  |2 NLM 
650 7 |a 27432CM55Q  |2 NLM 
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700 1 |a Bezerril, P M  |e verfasserin  |4 aut 
700 1 |a Dantas, T N C  |e verfasserin  |4 aut 
700 1 |a Pereira, M R  |e verfasserin  |4 aut 
700 1 |a Fonseca, J L C  |e verfasserin  |4 aut 
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