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231223s2007 xx ||||| 00| ||eng c |
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|a pubmed25n0569.xml
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|a (DE-627)NLM170663787
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|a (NLM)17545228
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Xu, Guo-Hua
|e verfasserin
|4 aut
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| 245 |
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|a Functional characterization of LePT4
|b a phosphate transporter in tomato with mycorrhiza-enhanced expression
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|c 2007
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 02.01.2008
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|a Date Revised 07.12.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Many plant roots acquire inorganic phosphate (Pi) from soils directly through the root-soil interface via high-affinity Pi transporters and/or through symbiotic associations between the cortical cells and arbuscular mycorrhizal fungi. In tomato, three phosphate transporters (LePT3, LePT4, and LePT5) are up-regulated upon colonization by arbuscular mycorrhizal fungi. In this study, the role of LePT4 in tomato is elucidated by molecular and physiological characterizations of a loss-of-function mutant lept4. In the absence of mycorrhizal infection and under solution-Pi concentrations (Cp) of 0.05 mM and 0.5 mM, the mutant exhibited severe Pi-deficiency symptoms which were associated with significantly lower Pi uptake as compared with that of the wild type. However, at a Cp of 5 mM, lept4 grew better than the wild type. Mycorrhizal infection at a Cp of 0.05 mM resulted in a significant increase in the transcripts of LePT4 in the wild type and a concomitant 2-fold increase in Pi uptake. Although upon mycorrhizal infection, lept4 also exhibited an increased Pi uptake, it was significantly lower than that of the wild type. Under a Cp of 1 mM and in the absence of mycorrhizal infection, LePT4 expression was suppressed in the wild type and a mutation in this gene resulted in a slight reduction in total Pi uptake. These data highlight the pivotal role of LePT4 in mycorrhizal-mediated Pi uptake in tomato, and show that this function may not be fully compensated by other members of the family. Characterization of the mycorrhiza-associated Pi transporter lept4 mutant, along with expression analysis of LePT3, provides evidence for the different routes of mycorrhiza-mediated Pi uptake in plants
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Phosphate Transport Proteins
|2 NLM
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| 650 |
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7 |
|a Phosphates
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Chague, Veronique
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Melamed-Bessudo, Cathy
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kapulnik, Yoram
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jain, Ajay
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Raghothama, Kashchandra G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Levy, Avraham A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Silber, Avner
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 58(2007), 10 vom: 14., Seite 2491-501
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:58
|g year:2007
|g number:10
|g day:14
|g pages:2491-501
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|a AR
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|d 58
|j 2007
|e 10
|b 14
|h 2491-501
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