Lateral diffusion coefficients of an eicosanyl-based bisglycerophosphocholine determined by PFG-NMR and FRAP

We report the lateral diffusion properties of 2,2'-di-O-decyl-3,3'-di-O-(eicosanyl)-bis-(rac-glycero)-1,1'-diphosphocholine (C20BAS) using pulsed-field gradient NMR (PFG-NMR) and fluorescence recovery after photobleaching (FRAP). C20BAS membranes display a melting transition at Tm = 1...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 23(2007), 11 vom: 22. Mai, Seite 6276-80
1. Verfasser: Febo-Ayala, Wilma (VerfasserIn)
Weitere Verfasser: Holland, David P, Bradley, Scott A, Thompson, David H
Format: Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Membrane Lipids Glycerylphosphorylcholine 60M22SGW66
LEADER 01000caa a22002652 4500
001 NLM169955672
003 DE-627
005 20240415232625.0
007 tu
008 231223s2007 xx ||||| 00| ||eng c
028 5 2 |a pubmed24n1376.xml 
035 |a (DE-627)NLM169955672 
035 |a (NLM)17465580 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Febo-Ayala, Wilma  |e verfasserin  |4 aut 
245 1 0 |a Lateral diffusion coefficients of an eicosanyl-based bisglycerophosphocholine determined by PFG-NMR and FRAP 
264 1 |c 2007 
336 |a Text  |b txt  |2 rdacontent 
337 |a ohne Hilfsmittel zu benutzen  |b n  |2 rdamedia 
338 |a Band  |b nc  |2 rdacarrier 
500 |a Date Completed 11.07.2007 
500 |a Date Revised 15.04.2024 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a We report the lateral diffusion properties of 2,2'-di-O-decyl-3,3'-di-O-(eicosanyl)-bis-(rac-glycero)-1,1'-diphosphocholine (C20BAS) using pulsed-field gradient NMR (PFG-NMR) and fluorescence recovery after photobleaching (FRAP). C20BAS membranes display a melting transition at Tm = 15.7 degrees C, as determined by differential scanning calorimetry and 31P NMR chemical shift anisotropy. The lateral diffusion coefficient of C20BAS, as determined by PFG-NMR and FRAP, at 25 degrees C, were DPFG-NMR = 1.9 +/- 0.6 x 10(-8) cm2/s and DFRAP C20BAS = 1.2 +/- 0.1 x 10(-8) cm2/s, respectively. In comparison, the lateral diffusion coefficient of the monopolar phospholipid, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), was 1.8 +/- 0.9 x 10(-8) and 2.5 +/- 0.9 x 10(-8) cm2/s using PFG-NMR and FRAP, respectively 
650 4 |a Journal Article 
650 4 |a Research Support, N.I.H., Extramural 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Membrane Lipids  |2 NLM 
650 7 |a Glycerylphosphorylcholine  |2 NLM 
650 7 |a 60M22SGW66  |2 NLM 
700 1 |a Holland, David P  |e verfasserin  |4 aut 
700 1 |a Bradley, Scott A  |e verfasserin  |4 aut 
700 1 |a Thompson, David H  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Langmuir : the ACS journal of surfaces and colloids  |d 1992  |g 23(2007), 11 vom: 22. Mai, Seite 6276-80  |w (DE-627)NLM098181009  |x 1520-5827  |7 nnns 
773 1 8 |g volume:23  |g year:2007  |g number:11  |g day:22  |g month:05  |g pages:6276-80 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_22 
912 |a GBV_ILN_350 
912 |a GBV_ILN_721 
951 |a AR 
952 |d 23  |j 2007  |e 11  |b 22  |c 05  |h 6276-80