Actin network formation by unidirectional polycation diffusion

We show that F-actins form three-dimensional giant network under uni-directional diffusion of polycations, at a dilute actin concentration (0.01 mg/mL) that only bundles are formed by homogeneous mixing with polycations. The mesh size of the actin network depends on polycation concentration and ioni...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 23(2007), 11 vom: 22. Mai, Seite 6257-62
1. Verfasser: Kwon, Hyuck Joon (VerfasserIn)
Weitere Verfasser: Kakugo, Akira, Ura, Takehiro, Okajima, Takaharu, Tanaka, Yoshimi, Furukawa, Hidemitsu, Osada, Yoshihito, Gong, Jian Ping
Format: Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Actins Biopolymers Cations Multiprotein Complexes
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520 |a We show that F-actins form three-dimensional giant network under uni-directional diffusion of polycations, at a dilute actin concentration (0.01 mg/mL) that only bundles are formed by homogeneous mixing with polycations. The mesh size of the actin network depends on polycation concentration and ionic strength, while bundle thickness of network depends only on ionic strength, which indicates that actin network is formed through nucleation-growth mechanism. The mesh size and the bundle thickness are determined by nucleus concentration and nucleus size, respectively. The atomic force microscopy measurement correlates the elasticity of the actin network, E, with the mesh size, xi, as E approximately xi-1, while the bundle thickness, D dependence of E cannot be described by a simple scaling relation. E approximately D6.5 when D is small and E approximately D0.1 when D is large. Our study on the self-assembly of actin network under asymmetric polycation condition would provide the crucial insight into the organization of biopolymers in polarized condition of cell 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Biopolymers  |2 NLM 
650 7 |a Cations  |2 NLM 
650 7 |a Multiprotein Complexes  |2 NLM 
700 1 |a Kakugo, Akira  |e verfasserin  |4 aut 
700 1 |a Ura, Takehiro  |e verfasserin  |4 aut 
700 1 |a Okajima, Takaharu  |e verfasserin  |4 aut 
700 1 |a Tanaka, Yoshimi  |e verfasserin  |4 aut 
700 1 |a Furukawa, Hidemitsu  |e verfasserin  |4 aut 
700 1 |a Osada, Yoshihito  |e verfasserin  |4 aut 
700 1 |a Gong, Jian Ping  |e verfasserin  |4 aut 
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