Using nucleation rates to determine the interfacial line tension of symmetric and asymmetric lipid bilayer domains

This work presents a novel method for experimentally quantifying interfacial line tension, which can be readily applied to study a wide variety of different lipid mixtures exhibiting phase coexistence. The method combines AFM imaging of lipid domain nucleation with classical nucleation theories. The...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 23(2007), 11 vom: 22. Mai, Seite 5875-7
1. Verfasser: Blanchette, Craig D (VerfasserIn)
Weitere Verfasser: Lin, Wan-Chen, Orme, Christine A, Ratto, Timothy V, Longo, Marjorie L
Format: Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Galactosylceramides Lipid Bilayers Phosphatidylcholines 1,2-distearoyllecithin EAG959U971 1,2-oleoylphosphatidylcholine EDS2L3ODLV
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245 1 0 |a Using nucleation rates to determine the interfacial line tension of symmetric and asymmetric lipid bilayer domains 
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520 |a This work presents a novel method for experimentally quantifying interfacial line tension, which can be readily applied to study a wide variety of different lipid mixtures exhibiting phase coexistence. The method combines AFM imaging of lipid domain nucleation with classical nucleation theories. The results, using symmetric and asymmetric domains, permit the prediction of key physical parameters (critical nuclei size and nucleation rate) in multicomponent bilayer systems with implications toward understanding the dynamic nature of submicrometer domains (i.e., lipid rafts) in cell membranes 
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650 4 |a Research Support, N.I.H., Extramural 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
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700 1 |a Orme, Christine A  |e verfasserin  |4 aut 
700 1 |a Ratto, Timothy V  |e verfasserin  |4 aut 
700 1 |a Longo, Marjorie L  |e verfasserin  |4 aut 
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