Specific adsorption of histidine-tagged proteins on silica surfaces modified with Ni2+/NTA-derivatized poly(ethylene glycol)

Silica surfaces modified with nitrilotriacetic acid (NTA)-polyethylene glycol (PEG) derivatives were used to immobilize hexahistidine-tagged green fluorescent protein (His6-GFP), biotin/streptavidin-AlexaFluor555 (His6-biotin/SA-AF), and gramicidin A-containing vesicles (His6-gA). Three types of sur...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 23(2007), 11 vom: 22. Mai, Seite 6281-8
1. Verfasser: Kang, Eunah (VerfasserIn)
Weitere Verfasser: Park, Jin-Won, McClellan, Scott J, Kim, Jong-Mok, Holland, David P, Lee, Gil U, Franses, Elias I, Park, Kinam, Thompson, David H
Format: Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Lipid Bilayers Proteins Polyethylene Glycols 3WJQ0SDW1A Histidine 4QD397987E Silicon Dioxide mehr... 7631-86-9 Nickel 7OV03QG267 Nitrilotriacetic Acid KA90006V9D