Localization of sphingomyelin in cholesterol domains by imaging mass spectrometry

The location of each lipid in a palmitoyloleoylphosphatidylcholine/18:0 sphingomyelin/cholesterol monolayer system is laterally resolved using imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) without the necessity of adding fluorescent labels. This system of coexisting immiscible li...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 23(2007), 10 vom: 08. Mai, Seite 5645-50
1. Verfasser: McQuaw, Carolyn M (VerfasserIn)
Weitere Verfasser: Zheng, Leiliang, Ewing, Andrew G, Winograd, Nicholas
Format: Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Membranes, Artificial Sphingomyelins Cholesterol 97C5T2UQ7J
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245 1 0 |a Localization of sphingomyelin in cholesterol domains by imaging mass spectrometry 
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520 |a The location of each lipid in a palmitoyloleoylphosphatidylcholine/18:0 sphingomyelin/cholesterol monolayer system is laterally resolved using imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) without the necessity of adding fluorescent labels. This system of coexisting immiscible liquid phases shows cholesterol domains with sizes and shapes comparable to those in the fluorescence microscopy literature. The results show that SM localizes with cholesterol and that palmitoyloleoylphosphatidylcholine is excluded. Moreover, the segregation is not complete, and there is a small amount of both phospholipids distributed throughout 
650 4 |a Journal Article 
650 4 |a Research Support, N.I.H., Extramural 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
650 7 |a Membranes, Artificial  |2 NLM 
650 7 |a Sphingomyelins  |2 NLM 
650 7 |a Cholesterol  |2 NLM 
650 7 |a 97C5T2UQ7J  |2 NLM 
700 1 |a Zheng, Leiliang  |e verfasserin  |4 aut 
700 1 |a Ewing, Andrew G  |e verfasserin  |4 aut 
700 1 |a Winograd, Nicholas  |e verfasserin  |4 aut 
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