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20231223121205.0 |
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231223s2007 xx ||||| 00| ||eng c |
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|a pubmed24n0565.xml
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|a (DE-627)NLM169367878
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|a (NLM)17402759
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wang, Xiqing
|e verfasserin
|4 aut
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| 245 |
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|a Protein refolding assisted by periodic mesoporous organosilicas
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|c 2007
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 27.06.2007
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|a Date Revised 01.12.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Herein we report a new strategy for protein refolding by taking advantage of the unique surface and pore characteristics of ethylene-bridged periodic mesoporous organosilica (PMO), which can effectively entrap unfolded proteins and assist refolding by controlled release into the refolding buffer. Hen egg white lysozyme was used as a model protein to demonstrate the new method of protein refolding. Through loading of denatured proteins inside uniform mesoporous channels tailored to accommodate individual protein, protein aggregation was minimized, and the folding rate was increased. Poly(ethyleneglycol) (PEG)-triggered continuous release of entrapped denatured lysozyme allowed high-yield refolding with high cumulative protein concentrations. The new method enhances the oxidative refolding of lysozyme (e.g., over 80% refolding yield at about 0.6 mg/mL)
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Silanes
|2 NLM
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|a Polyethylene Glycols
|2 NLM
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|a 3WJQ0SDW1A
|2 NLM
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|a tetraethoxysilane
|2 NLM
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| 650 |
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|a 42064KRE49
|2 NLM
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|a Silicon Dioxide
|2 NLM
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|a 7631-86-9
|2 NLM
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| 650 |
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|a Muramidase
|2 NLM
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|a EC 3.2.1.17
|2 NLM
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| 700 |
1 |
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|a Lu, Diannan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Austin, Rebecca
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Agarwal, Arun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mueller, Leonard J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Zheng
|e verfasserin
|4 aut
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| 700 |
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|a Wu, Jianzhong
|e verfasserin
|4 aut
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| 700 |
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|a Feng, Pingyun
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 23(2007), 10 vom: 08. Mai, Seite 5735-9
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:23
|g year:2007
|g number:10
|g day:08
|g month:05
|g pages:5735-9
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|a AR
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|d 23
|j 2007
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|h 5735-9
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