Influence of different cyto-stimuli on immunological activity of splenic regulatory T cells in mice
OBJECTIVE: To investigate the expression of cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and intranuclear forkhead/winged helix transcription factor p3 (Foxp3) of splenic regulatory T cells (Tregs) in mice, and compare the effects of different stimuli on the immunological activity of Tregs
| Veröffentlicht in: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue. - 1998. - 19(2007), 3 vom: 22. März, Seite 142-5 |
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| Weitere Verfasser: | , , |
| Format: | Aufsatz |
| Sprache: | Chinese |
| Veröffentlicht: |
2007
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| Zugriff auf das übergeordnete Werk: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue |
| Schlagworte: | Comparative Study Journal Article Research Support, Non-U.S. Gov't Antibodies Antigens, CD CD3 Complex CTLA-4 Antigen Ctla4 protein, mouse Forkhead Transcription Factors Foxp3 protein, mouse mehr... |
| Zusammenfassung: | OBJECTIVE: To investigate the expression of cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and intranuclear forkhead/winged helix transcription factor p3 (Foxp3) of splenic regulatory T cells (Tregs) in mice, and compare the effects of different stimuli on the immunological activity of Tregs METHODS: CD4+CD25+ Tregs isolated from the spleens of male BALB/c mice by magnetic beads were seeded on 96-well (1x10(5) cells/well) cell culture plates supplemented with phytohemagglutin (PHA, 20 mg/L), concanavalin A (ConA, 5 g/L) or coated with anti-CD3 (1 mg/L). After being stimulated with different stimuli for various length of time, the expressions of CTLA-4 and Foxp3 molecules of Tregs were determined. The time-dependent responses between different stimuli and CTLA-4 as well as Foxp3 were analyzed by means of flow cytometry RESULTS: After stimulation with PHA, the CTLA-4 expressions on surface of mice splenic Tregs and intranuclear Foxp3 molecules were unchanged (both P>0.05). In ConA-treated group, the expression of CTLA-4 was significantly up-regulated at 24 hours (P<0.01), while the CTLA-4 expression returned to the control range at 48 and 72 hours. The expression of Foxp3 was not markedly different between ConA-treated group and controls. When treated with plate-bound anti-CD3, both CTLA-4 and Foxp3 expressions were significantly up-regulated at 24, 48 and 72 hours (P<0.05 or P<0.01), especially at 24 and 48 hours (all P<0.01) CONCLUSION: These data indicated that various stimuli can result in different modulatory responses of the CTLA-4 and Foxp3 expressions in mice splenic Tregs. Anti-CD3 appears to be an effective immunoregulatory signal that influences the activation of CD4+CD25+ Tregs |
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| Beschreibung: | Date Completed 09.07.2009 Date Revised 16.11.2017 published: Print Citation Status MEDLINE |
| ISSN: | 1003-0603 |