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01000naa a22002652 4500 |
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NLM169040453 |
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DE-627 |
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20231223120454.0 |
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231223s2007 xx ||||| 00| ||eng c |
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|a pubmed24n0564.xml
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|a (DE-627)NLM169040453
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|a (NLM)17367172
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Petkova, Vera
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Free-standing films of fluorinated surfactants as 2D matrices for organizing detergent-solubilized membrane proteins
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| 264 |
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1 |
|c 2007
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 27.06.2007
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The possibility of organizing detergent-solubilized membrane proteins in a plane within the core of Newton black films (NBFs) formed from fluorinated surfactants has been investigated. Fluorinated surfactants have the interesting characteristics of being poorly miscible with detergents and highly surface-active. As a result, when a membrane protein-the transmembrane domain of OmpA (tOmpA)-solubilized by the nonionic detergent C8E4 (tetraethylene glycol monooctyl ether) was injected under a monolayer of fluorinated surfactant, C8E4 and tOmpA/C8E4 complexes remained confined to the subphase. Vertical, macroscopic NBFs were drawn, and their structure was investigated by means of X-ray reflectivity. Depending on experimental conditions, the protein was shown to organize into either one or two monolayers stabilized by two monolayers of fluorinated surfactant. Two different mechanisms of protein insertion were investigated: (i) attachment of polyhistidine-tagged tOmpA/C8E4 complexes to nickel-bearing polar groups born by a fluorinated surfactant and (ii) spontaneous diffusion into the surfactant films. Possible applications are discussed
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| 650 |
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4 |
|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Bacterial Outer Membrane Proteins
|2 NLM
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| 650 |
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7 |
|a Detergents
|2 NLM
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| 650 |
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7 |
|a Lipoproteins
|2 NLM
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| 650 |
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7 |
|a Membrane Proteins
|2 NLM
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| 650 |
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|a Micelles
|2 NLM
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| 650 |
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7 |
|a Proteins
|2 NLM
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| 650 |
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|a Surface-Active Agents
|2 NLM
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| 650 |
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|a OMPA outer membrane proteins
|2 NLM
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| 650 |
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7 |
|a 149024-69-1
|2 NLM
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| 650 |
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7 |
|a polyhistidine
|2 NLM
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| 650 |
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|a 26062-48-6
|2 NLM
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| 650 |
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|a Fluorine
|2 NLM
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| 650 |
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7 |
|a 284SYP0193
|2 NLM
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| 650 |
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7 |
|a Histidine
|2 NLM
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| 650 |
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7 |
|a 4QD397987E
|2 NLM
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| 650 |
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7 |
|a Nickel
|2 NLM
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| 650 |
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7 |
|a 7OV03QG267
|2 NLM
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| 700 |
1 |
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|a Benattar, Jean-Jacques
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zoonens, Manuela
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zito, Francesca
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Popot, Jean-Luc
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Polidori, Ange
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jasseron, Sylvain
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pucci, Bernard
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 23(2007), 8 vom: 10. Apr., Seite 4303-9
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
8 |
|g volume:23
|g year:2007
|g number:8
|g day:10
|g month:04
|g pages:4303-9
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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|a GBV_ILN_22
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| 912 |
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|a GBV_ILN_350
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| 912 |
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|a GBV_ILN_721
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| 951 |
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|a AR
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| 952 |
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|d 23
|j 2007
|e 8
|b 10
|c 04
|h 4303-9
|