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NLM168955830 |
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DE-627 |
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20231223120255.0 |
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231223s2007 xx ||||| 00| ||eng c |
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|a pubmed24n0563.xml
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|a (DE-627)NLM168955830
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|a (NLM)17358090
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Kusumo, Andy
|e verfasserin
|4 aut
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| 245 |
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|a High capacity, charge-selective protein uptake by polyelectrolyte brushes
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|c 2007
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 27.06.2007
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|a Date Revised 16.11.2017
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Surface plasmon resonance was used to measure binding of proteins from solution to poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) brushes end-grafted from gold surfaces by atom transfer radical polymerization (ATRP). PDMAEMA brushes were prepared with a variety of grafting densities and degrees of polymerization. These brushes displayed charge selective protein uptake. The extent of uptake for net negatively charged bovine serum albumin (BSA) scaled linearly with the surface mass concentration of grafted PDMAEMA, regardless of grafting density. BSA was bound at a constant ratio of 120 DMAEMA monomer units per protein molecule for all brushes examined. The equivalent three-dimensional concentration of BSA bound in the brush (i.e., the bound BSA surface excess concentration divided by the brush thickness) decreased monotonically with decreasing grafting density. The concentration of BSA bound within brushes prepared at higher grafting densities was comparable with the aqueous protein solubility limit. BSA desorption from the brush required changes in solution pH and/or ionic strength to eliminate its net electrostatic attraction to PDMAEMA. Net positively charged lysozyme was completely rejected by the PDMAEMA brushes
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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7 |
|a Electrolytes
|2 NLM
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| 650 |
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7 |
|a Ions
|2 NLM
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|a Methacrylates
|2 NLM
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|a Nylons
|2 NLM
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7 |
|a Polymers
|2 NLM
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|a Proteins
|2 NLM
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|a poly(2-(dimethylamino)ethyl methacrylate)
|2 NLM
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|a Serum Albumin, Bovine
|2 NLM
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| 650 |
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7 |
|a 27432CM55Q
|2 NLM
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| 650 |
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7 |
|a Gold
|2 NLM
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| 650 |
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7 |
|a 7440-57-5
|2 NLM
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| 650 |
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|a Muramidase
|2 NLM
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| 650 |
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|a EC 3.2.1.17
|2 NLM
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| 700 |
1 |
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|a Bombalski, Lindsay
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lin, Qiao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Matyjaszewski, Krzysztof
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schneider, James W
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tilton, Robert D
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 23(2007), 8 vom: 10. Apr., Seite 4448-54
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
8 |
|g volume:23
|g year:2007
|g number:8
|g day:10
|g month:04
|g pages:4448-54
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_22
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|a GBV_ILN_350
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|a GBV_ILN_721
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| 951 |
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|a AR
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| 952 |
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|d 23
|j 2007
|e 8
|b 10
|c 04
|h 4448-54
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