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231223s2007 xx ||||| 00| ||eng c |
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|a pubmed24n0563.xml
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|a (DE-627)NLM168823705
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|a (NLM)17344056
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Johnston, Jason W
|e verfasserin
|4 aut
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| 245 |
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|a Evaluation of the 1-methyl-2-phenylindole colorimetric assay for aldehydic lipid peroxidation products in plants
|b malondialdehyde and 4-hydroxynonenal
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|c 2007
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 18.06.2007
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The 1-methyl-2-phenylindole colorimetric assay is considered specific for malondialdehyde (MDA) and 4-hydroxynonenal (HNE) in mammalian systems, but its specificity in plant tissues is unknown. This study demonstrates that the assay produces a purple/blue chromophore with an absorbance peak at 586 nm for a malondialdehyde standard, while aqueous extractions from Ribes spp. Beta vulgaris, and Lycopersicon esculentum tissues produce an orange chromophore with an absorbance maximum at 450 nm and a large shoulder that extends to 700 nm. No distinctive MDA peak was discernable in plant samples at lambda=586 nm and absorbance was attributed to background interference. The reaction between sucrose and 1-methyl-2-phenylindole produced an orange chromophore with a spectrum similar to those obtained from plant extractions, suggesting that simple sugars are the likely source of background interference. This study demonstrates that the 1-methyl-2-phenylindole colorimetric assay is non-specific for detecting MDA and HNE in plants and its use is cautioned due to interference, particularly from sugars
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Aldehydes
|2 NLM
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|a Indoles
|2 NLM
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|a Mesylates
|2 NLM
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|a Plant Proteins
|2 NLM
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|a methanesulfonic acid
|2 NLM
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|a 12EH9M7279
|2 NLM
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|a Malondialdehyde
|2 NLM
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|a 4Y8F71G49Q
|2 NLM
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|a 1-methyl-2-phenylindole
|2 NLM
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|a IE3UB978IA
|2 NLM
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|a 4-hydroxy-2-nonenal
|2 NLM
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|a K1CVM13F96
|2 NLM
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| 650 |
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|a Hydrochloric Acid
|2 NLM
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| 650 |
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|a QTT17582CB
|2 NLM
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| 700 |
1 |
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|a Horne, Susan
|e verfasserin
|4 aut
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|a Harding, Keith
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Benson, Erica E
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 45(2007), 2 vom: 01. Feb., Seite 108-12
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
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|g volume:45
|g year:2007
|g number:2
|g day:01
|g month:02
|g pages:108-12
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a AR
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|d 45
|j 2007
|e 2
|b 01
|c 02
|h 108-12
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