Electrochemical evaluation of self-disassociation of PKA upon activation by cAMP
The allosteric reaction of protein kinase A (PKA) upon binding of cyclic AMP (cAMP) is revealed with an electrochemical technique through the redox current change of an electrochemically active marker. The different effect of cAMP's regulation at a distinct concentration level is obtained in th...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 23(2007), 7 vom: 27. März, Seite 3506-8 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2007
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Ligands Cyclic AMP E0399OZS9N Cyclic AMP-Dependent Protein Kinases EC 2.7.11.11 |
| Zusammenfassung: | The allosteric reaction of protein kinase A (PKA) upon binding of cyclic AMP (cAMP) is revealed with an electrochemical technique through the redox current change of an electrochemically active marker. The different effect of cAMP's regulation at a distinct concentration level is obtained in this system. The influence of structural analogues is also examined with respect to the affinity and special selectivity. This study presents an electrochemical approach to the rapid and sensitive investigation of the protein-ligand interaction in the signal transduction networks |
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| Beschreibung: | Date Completed 15.05.2007 Date Revised 21.11.2013 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |