|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM168218135 |
| 003 |
DE-627 |
| 005 |
20231223114634.0 |
| 007 |
tu |
| 008 |
231223s2007 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed24n0561.xml
|
| 035 |
|
|
|a (DE-627)NLM168218135
|
| 035 |
|
|
|a (NLM)17279684
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Naves, Alliny F
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Immobilized horseradish peroxidase as a reusable catalyst for emulsion polymerization
|
| 264 |
|
1 |
|c 2007
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 02.05.2007
|
| 500 |
|
|
|a Date Revised 21.11.2013
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a The study on the adsorption of horseradish peroxidase (HRP) onto silicon wafers was carried out by means of in situ ellipsometry, atomic force microscopy (AFM) and contact angle measurements. A smooth HRP layer adsorbed onto Si wafers. The enzymatic activity of free or adsorbed HRP was determined by the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and by the emulsion polymerization of ethylene glycol dimethacrylate (EGDMA). Upon adsorbing, HRP molecules might have undergone some conformational changes, which caused a small reduction of enzymatic activity in comparison to that observed for HRP solution. However, it was possible to reuse the same HRP-covered Si wafer as catalyst in the polymerization of EGDMA three times
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a Emulsions
|2 NLM
|
| 650 |
|
7 |
|a Enzymes, Immobilized
|2 NLM
|
| 650 |
|
7 |
|a Free Radicals
|2 NLM
|
| 650 |
|
7 |
|a Methacrylates
|2 NLM
|
| 650 |
|
7 |
|a Polymers
|2 NLM
|
| 650 |
|
7 |
|a ethylene dimethacrylate
|2 NLM
|
| 650 |
|
7 |
|a 7BK5G69305
|2 NLM
|
| 650 |
|
7 |
|a Hydrogen Peroxide
|2 NLM
|
| 650 |
|
7 |
|a BBX060AN9V
|2 NLM
|
| 650 |
|
7 |
|a Horseradish Peroxidase
|2 NLM
|
| 650 |
|
7 |
|a EC 1.11.1.-
|2 NLM
|
| 650 |
|
7 |
|a Silicon
|2 NLM
|
| 650 |
|
7 |
|a Z4152N8IUI
|2 NLM
|
| 700 |
1 |
|
|a Carmona-Ribeiro, Ana M
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Petri, Denise F S
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1999
|g 23(2007), 4 vom: 13. Feb., Seite 1981-7
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:23
|g year:2007
|g number:4
|g day:13
|g month:02
|g pages:1981-7
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 23
|j 2007
|e 4
|b 13
|c 02
|h 1981-7
|