|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM167894846 |
| 003 |
DE-627 |
| 005 |
20231223113936.0 |
| 007 |
tu |
| 008 |
231223s2007 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed24n0560.xml
|
| 035 |
|
|
|a (DE-627)NLM167894846
|
| 035 |
|
|
|a (NLM)17244632
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Tomassen, Monic M M
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Isolation and characterization of a tomato non-specific lipid transfer protein involved in polygalacturonase-mediated pectin degradation
|
| 264 |
|
1 |
|c 2007
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 11.07.2007
|
| 500 |
|
|
|a Date Revised 07.12.2022
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a An important aspect of the ripening process of tomato fruit is softening. Softening is accompanied by hydrolysis of the pectin in the cell wall by pectinases, causing loss of cell adhesion in the middle lamella. One of the most significant pectin-degrading enzymes is polygalacturonase (PG). Previous reports have shown that PG in tomato may exist in different forms (PG1, PG2a, PG2b, and PGx) commonly referred to as PG isoenzymes. The gene product PG2 is differentially glycosylated and is thought to associate with other proteins to form PG1 and PGx. This association is thought to modulate its pectin-degrading activity in planta. An 8 kDa protein that is part of the tomato PG1 multiprotein complex has been isolated, purified, and functionally characterized. This protein, designated 'activator' (ACT), belongs to the class of non-specific lipid transfer proteins (nsLTPs). ACT is capable of 'converting' the gene product PG2 into a more active and heat-stable form, which increases PG-mediated pectin degradation in vitro and stimulates PG-mediated tissue breakdown in planta. This finding suggests a new, not previously identified, function for nsLTPs in the modification of hydrolytic enzyme activity. It is proposed that ACT plays a role in the modulation of PG activity during tomato fruit softening
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
7 |
|a Carrier Proteins
|2 NLM
|
| 650 |
|
7 |
|a Isoenzymes
|2 NLM
|
| 650 |
|
7 |
|a lipid transfer protein
|2 NLM
|
| 650 |
|
7 |
|a Pectins
|2 NLM
|
| 650 |
|
7 |
|a 89NA02M4RX
|2 NLM
|
| 650 |
|
7 |
|a Polygalacturonase
|2 NLM
|
| 650 |
|
7 |
|a EC 3.2.1.15
|2 NLM
|
| 700 |
1 |
|
|a Barrett, Diane M
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a van der Valk, Henry C P M
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Woltering, Ernst J
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 58(2007), 5 vom: 23., Seite 1151-60
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:58
|g year:2007
|g number:5
|g day:23
|g pages:1151-60
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 58
|j 2007
|e 5
|b 23
|h 1151-60
|