Modification of nitrogen remobilization, grain fill and leaf senescence in maize (Zea mays) by transposon insertional mutagenesis in a protease gene

A maize (Zea mays) senescence-associated legumain gene, See2beta, was characterized at the physiological and molecular levels to determine its role in senescence and resource allocation. A reverse-genetics screen of a maize Mutator (Mu) population identified a Mu insertion in See2beta. Maize plants...

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Veröffentlicht in:The New phytologist. - 1979. - 173(2007), 3 vom: 31., Seite 481-494
1. Verfasser: Donnison, Iain S (VerfasserIn)
Weitere Verfasser: Gay, Alan P, Thomas, Howard, Edwards, Keith J, Edwards, David, James, Caron L, Thomas, Ann M, Ougham, Helen J
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA Transposable Elements DNA, Plant RNA, Messenger Chlorophyll 1406-65-1 Carotenoids 36-88-4 Peptide Hydrolases mehr... EC 3.4.- Nitrogen N762921K75
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100 1 |a Donnison, Iain S  |e verfasserin  |4 aut 
245 1 0 |a Modification of nitrogen remobilization, grain fill and leaf senescence in maize (Zea mays) by transposon insertional mutagenesis in a protease gene 
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520 |a A maize (Zea mays) senescence-associated legumain gene, See2beta, was characterized at the physiological and molecular levels to determine its role in senescence and resource allocation. A reverse-genetics screen of a maize Mutator (Mu) population identified a Mu insertion in See2beta. Maize plants homozygous for the insertion were produced. These See2 mutant and sibling wild-type plants were grown under high or low quantities of nitrogen (N). The early development of both genotypes was similar; however, tassel tip and collar emergence occurred earlier in the mutant. Senescence of the mutant leaves followed a similar pattern to that of wild-type leaves, but at later sampling points mutant plants contained more chlorophyll than wild-type plants and showed a small extension in photosynthetic activity. Total plant weight was higher in the wild-type than in the mutant, and there was a genotype x N interaction. Mutant plants under low N maintained cob weight, in contrast to wild-type plants under the same treatment. It is concluded, on the basis of transposon mutagenesis, that See2beta has an important role in N-use and resource allocation under N-limited conditions, and a minor but significant function in the later stages of senescence 
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650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Carotenoids  |2 NLM 
650 7 |a 36-88-4  |2 NLM 
650 7 |a Peptide Hydrolases  |2 NLM 
650 7 |a EC 3.4.-  |2 NLM 
650 7 |a Nitrogen  |2 NLM 
650 7 |a N762921K75  |2 NLM 
700 1 |a Gay, Alan P  |e verfasserin  |4 aut 
700 1 |a Thomas, Howard  |e verfasserin  |4 aut 
700 1 |a Edwards, Keith J  |e verfasserin  |4 aut 
700 1 |a Edwards, David  |e verfasserin  |4 aut 
700 1 |a James, Caron L  |e verfasserin  |4 aut 
700 1 |a Thomas, Ann M  |e verfasserin  |4 aut 
700 1 |a Ougham, Helen J  |e verfasserin  |4 aut 
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