Effects of macroporous resin size on Candida antarctica lipase B adsorption, fraction of active molecules, and catalytic activity for polyester synthesis
Methyl methacrylate resins with identical average pore diameter (250 A) and surface area (500 m2/g) but with varied particle size (35 to 560-710 microm) were employed to study how immobilization resin particle size influences Candida antarctica Lipase B (CALB) loading, fraction of active sites, and...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 23(2007), 3 vom: 30. Jan., Seite 1381-7 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2007
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Enzymes, Immobilized Fungal Proteins Polyesters Resins, Synthetic Lipase EC 3.1.1.3 lipase B, Candida antarctica |
| Zusammenfassung: | Methyl methacrylate resins with identical average pore diameter (250 A) and surface area (500 m2/g) but with varied particle size (35 to 560-710 microm) were employed to study how immobilization resin particle size influences Candida antarctica Lipase B (CALB) loading, fraction of active sites, and catalytic properties for polyester synthesis. CALB adsorbed more rapidly on smaller beads. Saturation occurred in less than 30 s and 48 h for beads with diameters 35 and 560-710 microm, respectively. Linearization of adsorption isotherm data by the Scatchard analysis showed for the 35 microm resin that: (i) CALB loading at saturation was well below that required to form a monolayer and fully cover the support surface and (ii) CALB has a high affinity for this resin surface. Infrared microspectroscopy showed that CALB forms protein loading fronts for resins with particle sizes 560-710 and 120 microm. In contrast, CALB appears evenly distributed throughout 35 microm resins. By titration with p-nitrophenyl n-hexyl phosphate (MNPHP), the fraction of active CALB molecules adsorbed onto resins was <50% which was not influenced by particle size. The fraction of active CALB molecules on the 35 microm support increased from 30 to 43% as enzyme loading was increased from 0.9 to 5.7% (w/w) leading to increased activity for epsilon-caprolactone (epsilon-CL) ring-opening polymerization. At about 5% w/w CALB loading, by decreasing the immobilization support diameter from 560-710 to 120, 75, and 35 microm, conversion of epsilon-CL % to polyester increased (20 to 36, 42, and 61%, respectively, at 80 min). Similar trends were observed for condensation polymerizations between 1,8-octanediol and adipic acid |
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| Beschreibung: | Date Completed 28.02.2007 Date Revised 24.11.2016 published: Print Citation Status MEDLINE |
| ISSN: | 1520-5827 |