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NLM167860755 |
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DE-627 |
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20250207232157.0 |
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tu |
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231223s2007 xx ||||| 00| ||eng c |
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2 |
|a pubmed25n0560.xml
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|a (DE-627)NLM167860755
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| 035 |
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|a (NLM)17241060
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Suo, Zhiyong
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a HEPES-stabilized encapsulation of Salmonella typhimurium
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| 264 |
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1 |
|c 2007
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 28.02.2007
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| 500 |
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a Most bacteria, planktonic and sessile, are encapsulated inside loosely bound extracellular polymeric substance (EPS) in their physiological environment. Imaging a bacterium with its capsule requires lengthy sample preparation to enhance the capsular contrast. In this study, Salmonella typhimurium was investigated using atomic force microscopy for a practical means of imaging an encapsulated bacterium in air. The investigation further aimed to determine the relation between the buffers used for preparing the bacterium and the preservation of the capsular material surrounding it. It was observed that rinsing bacteria with HEPES buffer could stabilize and promote capsule formation, while rinsing with PBS, Tris, or glycine removes most of the capsular EPS. For bacteria rinsed with HEPES and air-dried, the height images showed only the contour of the capsular material, while the phase and amplitude images presented the detailed structures of the bacterial surface, including the flagella encapsulated inside the capsular EPS. The encapsulation was attributed to the cross-linking of the acidic exopolysaccharides mediated by the piperazine moiety of HEPES through electrostatic attraction. This explanation is supported by encapsulated bacteria observed for samples rinsed with N,N'-bis(2-hydroxyethyl)-piperazine solution and by the presence of entrapped HEPES within the dry capsular EPS suggested by micro-Raman spectroscopy
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, N.I.H., Extramural
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| 650 |
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4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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7 |
|a Polysaccharides
|2 NLM
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| 650 |
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7 |
|a HEPES
|2 NLM
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| 650 |
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7 |
|a RWW266YE9I
|2 NLM
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| 700 |
1 |
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|a Yang, Xinghong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Avci, Recep
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kellerman, Laura
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pascual, David W
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fries, Marc
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Steele, Andrew
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1991
|g 23(2007), 3 vom: 30. Jan., Seite 1365-74
|w (DE-627)NLM098181009
|x 0743-7463
|7 nnns
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| 773 |
1 |
8 |
|g volume:23
|g year:2007
|g number:3
|g day:30
|g month:01
|g pages:1365-74
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_22
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| 912 |
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|a GBV_ILN_350
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| 912 |
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|a GBV_ILN_721
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| 951 |
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|a AR
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| 952 |
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|d 23
|j 2007
|e 3
|b 30
|c 01
|h 1365-74
|