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231223s2007 xx ||||| 00| ||eng c |
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|a pubmed24n0559.xml
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|a (DE-627)NLM167584790
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|a (NLM)17211079
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Ravelli, Raimond B G
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Plastic-embedded protein crystals
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|c 2007
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 06.03.2007
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|a Date Revised 09.01.2007
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Rapid vitrification followed by the replacement of the vitrified water by a solvent (freeze substitution) and then resin is a widely used procedure for preparing biological samples for electron microscopy. The resulting plastic-embedded samples permit convenient room-temperature sectioning (microtomy) and can yield well preserved cellular structures. Here this procedure has been applied to crystalline protein samples, and it is shown that it is possible to freeze-substitute vitrified crystals while preserving some of their original diffraction properties. The plastic-embedded crystals were used to collect a series of complete room-temperature data sets at a powerful macromolecular crystallography synchrotron beamline. Whereas one normally observes specific damage to disulfide bonds upon X-ray radiation, no such damage was seen for the plastic-embedded sample. The X-ray diffraction data allowed an initial atomic analysis to be made of the effects of freeze-substitution and plastic embedding on biological samples
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Acrylic Resins
|2 NLM
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| 650 |
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|a Coated Materials, Biocompatible
|2 NLM
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| 650 |
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|a hen egg lysozyme
|2 NLM
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| 650 |
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|a EC 3.2.1.-
|2 NLM
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| 650 |
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|a Muramidase
|2 NLM
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|a EC 3.2.1.17
|2 NLM
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| 700 |
1 |
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|a Haselmann-Weiss, Uta
|e verfasserin
|4 aut
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| 700 |
1 |
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|a McGeehan, John E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a McCarthy, Andrew A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Marquez, Josan A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Antony, Claude
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Frangakis, Achilleas S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Stranzl, Gudrun
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of synchrotron radiation
|d 1994
|g 14(2007), Pt 1 vom: 15. Jan., Seite 128-32
|w (DE-627)NLM09824129X
|x 1600-5775
|7 nnns
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| 773 |
1 |
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|g volume:14
|g year:2007
|g number:Pt 1
|g day:15
|g month:01
|g pages:128-32
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_ILN_40
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|a GBV_ILN_350
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|a GBV_ILN_2005
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|a AR
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|d 14
|j 2007
|e Pt 1
|b 15
|c 01
|h 128-32
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