X-ray radiation-induced damage in DNA monitored by online Raman

High-quality Raman spectra have been collected on single DNA crystals kept at 100 K in a flow of cold nitrogen gas prior, during and after macromolecular crystallography (MX) data collection. The non-symmetric bending vibration for C-Br bonds in 8-bromo-2'-deoxyguanosine has been unambiguously...

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Veröffentlicht in:Journal of synchrotron radiation. - 1994. - 14(2007), Pt 1 vom: 15. Jan., Seite 99-108
1. Verfasser: McGeehan, John E (VerfasserIn)
Weitere Verfasser: Carpentier, Philippe, Royant, Antoine, Bourgeois, Dominique, Ravelli, Raimond B G
Format: Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:Journal of synchrotron radiation
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA 9007-49-2
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520 |a High-quality Raman spectra have been collected on single DNA crystals kept at 100 K in a flow of cold nitrogen gas prior, during and after macromolecular crystallography (MX) data collection. The non-symmetric bending vibration for C-Br bonds in 8-bromo-2'-deoxyguanosine has been unambiguously identified at 293 cm(-1) in the non-resonant Raman spectra. The breakage of this bond could be monitored by the continuous recording of Raman spectra during X-ray exposure, and its decay versus dose could be directly correlated with the loss of the bromide definition within the electron-density maps as determined with MX 
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