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231223s2007 xx ||||| 00| ||eng c |
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|a (NLM)17209573
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|c DE-627
|e rakwb
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|a eng
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|a Hase, M
|e verfasserin
|4 aut
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| 245 |
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|a Manipulation of cell-sized phospholipid-coated microdroplets and their use as biochemical microreactors
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|c 2007
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 17.07.2007
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a Cell-sized water droplets coated by a phospholipid layer mimicking the inner surface of living cells were manipulated by laser tweezers and used as biochemical microreactors. The cell-sized phospholipid-coated microdroplets (CPMDs) consisted of a water droplet in mineral oil with a diameter of 1-100 microm and coated by 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine. We monitored the time development of biochemical reactions in a single CPMD obtained after the controlled fusion of two CPMDs containing a substrate and an enzyme, respectively. We present results on two enzymatic reactions: calcein production in the presence of esterase and green fluorescence protein expression
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Enzymes
|2 NLM
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|a Fluoresceins
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|a Liposomes
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|a Phospholipids
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|a Water
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|a 059QF0KO0R
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|a Green Fluorescent Proteins
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|a 147336-22-9
|2 NLM
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|a fluorexon
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|a Yamada, A
|e verfasserin
|4 aut
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|a Hamada, T
|e verfasserin
|4 aut
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|a Baigl, D
|e verfasserin
|4 aut
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|a Yoshikawa, K
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 23(2007), 2 vom: 16. Jan., Seite 348-52
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:23
|g year:2007
|g number:2
|g day:16
|g month:01
|g pages:348-52
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