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|a pubmed25n0559.xml
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|a (DE-627)NLM167549898
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|a (NLM)17207362
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Wang, Xiao-hui
|e verfasserin
|4 aut
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| 245 |
1 |
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|a p38 MAPK/cPLA2 pathway mediates interleukins release in inflammatory cell model
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|c 2007
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 07.07.2009
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|a Date Revised 19.11.2009
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a OBJECTIVE: To explore the underlying mechanism of lipopolysaccharide (LPS)-induced interleukin-1 beta (IL-1 beta) and IL-6 release via p38 mitogen-activated protein kinase (MAPK) pathway in HeLa cells for further identification of involved down-stream message factors
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|a METHODS: HeLa cells were challenged with LPS to reproduce inflammatory cell model. The activity or expression of p38 MAPK, cytosolic phospholipase A(2) (cPLA(2)) and COX-2, was inhibited with pretreatment of inflammatory HeLa cells with the inhibitors (SB203580, AACOCF(3), NS-398) or transfected with the cPLA(2) antisense oligonucleotide (SK7111), then the activities and/or expression of p38 MAPK, cPLA(2), COX-2, and relationship with levels of IL-1 beta and IL-6 supernatants were determined in each group
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|a RESULTS: SB203580 obviously down-regulated the activities of p38 and cPLA(2), as well as the release of IL-1 beta and IL-6. AACOCF(3) and SK7111 blocked dose-dependently the activity or expression of cPLA(2), IL-1 beta and IL-6 production. However, the expression of COX-2 could hardly be detected in HeLa cells, even after LPS treatment. At the same time, pre-treatment with NS-398 had no effect on IL-1 beta, IL-6 production
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|a CONCLUSION: p38 MAPK/cPLA(2) pathway mediates the expression of IL-1 beta and IL-6 resulting from LPS treatment of HeLa cells, while COX-2, as a down-stream enzyme of cPLA(2) has no effect in this process
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|a English Abstract
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Interleukin-1beta
|2 NLM
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| 650 |
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|a Interleukin-6
|2 NLM
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| 650 |
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|a Lipopolysaccharides
|2 NLM
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| 650 |
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|a Cyclooxygenase 2
|2 NLM
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| 650 |
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|a EC 1.14.99.1
|2 NLM
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| 650 |
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|a PTGS2 protein, human
|2 NLM
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| 650 |
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7 |
|a EC 1.14.99.1
|2 NLM
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| 650 |
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|a p38 Mitogen-Activated Protein Kinases
|2 NLM
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| 650 |
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|a EC 2.7.11.24
|2 NLM
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| 650 |
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|a Phospholipases A2, Cytosolic
|2 NLM
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| 650 |
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|a EC 3.1.1.4
|2 NLM
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| 700 |
1 |
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|a Yan, Guang-tao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Kai
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xue, Hui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hao, Xiu-hua
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Lu-huan
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 19(2007), 1 vom: 08. Jan., Seite 31-5
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
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|g volume:19
|g year:2007
|g number:1
|g day:08
|g month:01
|g pages:31-5
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 19
|j 2007
|e 1
|b 08
|c 01
|h 31-5
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