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NLM167162896 |
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DE-627 |
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20250207205948.0 |
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231223s2006 xx ||||| 00| ||chi c |
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|a pubmed25n0557.xml
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|a (DE-627)NLM167162896
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|a (NLM)17166346
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Bi, Ming-hua
|e verfasserin
|4 aut
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|a Effect of different fat emulsions on acute lung injury induced by endotoxin
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|c 2006
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 06.06.2011
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|a Date Revised 19.11.2015
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|a published: Print
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|a Citation Status MEDLINE
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|a OBJECTIVE: To assess the effect of Clinoleic 20% (olive oil-based, n-9) and Lipoven 20% (soy bean-based, n-6) lipid emulsions on inflammatory parameters in a murine acute lung injury (ALI) model induced by lipopolysaccharide (LPS) of E. coli O111:B4
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|a METHODS: Male Balb/C mice were infused for three days with 0.9% NaCl, Clinoleic 20%, or Lipoven 20% respectively, and sacrificed either at 8 hours or 24 hours after intra-tracheal introduction of LPS. Survival rate, lung wet/dry weight ratio (W/D), lung tissue myeloperoxidase (MPO) activity were determined, and tumor necrosis factor-alpha (TNF-alpha) and macrophage inflammatory protein-2 (MIP-2) in bronchoalveolar lavage fluid (BALF) were determined with enzyme linked immunosorbent assay (ELISA). Serum free fatty acids [arachidonic acid (AA), oleic acid, linoleic acid] were determined by gas chromatography. Leukocytes in BALF were counted under light microscope
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|a RESULTS: Lipoven significantly decreased survival rate at 24 hours after intra-tracheal LPS challenge compared to corresponding controls (both P<0.01). No significant difference was observed between Clinoleic and NaCl groups. MPO activity was obviously increased in lipids groups than that in NaCl group at 24 hours (both P<0.01), and no difference was found between two lipids groups. LPS markedly induced an increase in leukocyte infiltration, W/D ratio, lung MPO activity, release of TNF-alpha as well as MIP-2 into alveolar space in both lipids and NaCl groups. Pre-infusion with Lipoven gave rise to heavier leukocyte infiltration at 24 hours, which was blunted in Clinoleic group and NaCl group (both P<0.01). In contrast to Clinoleic and NaCl groups, Lipoven increased production of TNF-alpha at 24 hours and MIP-2 at 8 hours in LPS-treated mice (all P<0.01). Notably, lipid emulsions increased LPS-induced MPO activity, but no difference in effects was found in both Lipoven and Clinoleic groups. Clinoleic significantly reduced free AA at 8 and 24 hours compared with Lipoven (both P<0.01). There were no differences in lung tissues edema, serum oleic acid and linoleic acid among three groups
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|a CONCLUSION: In murine model of ALI, although LPS caused an increase in alveolar leucocytic infiltration, MPO activity, cytokine generation in both lipids and NaCl groups, Lipoven 20%, n-6 lipid emulsion induces a severer inflammatory reaction. It is speculated that by increasing AA, Lipoven 20% may aggravate ALI, whereas Clinoleic 20%, n-9 lipid emulsion possibly offers an alternative choice in producing less impact on inflammatory lung injury
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|a Comparative Study
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|a English Abstract
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Chemokine CXCL2
|2 NLM
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|a Dietary Fats, Unsaturated
|2 NLM
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|a Emulsions
|2 NLM
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|a Lipopolysaccharides
|2 NLM
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|a Olive Oil
|2 NLM
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| 650 |
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|a Plant Oils
|2 NLM
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| 650 |
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|a Tumor Necrosis Factor-alpha
|2 NLM
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|a Soybean Oil
|2 NLM
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| 650 |
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|a 8001-22-7
|2 NLM
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1 |
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|a Wang, Bao-en
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schafer, Martina
|e verfasserin
|4 aut
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| 700 |
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|a Mayer, Konstantin
|e verfasserin
|4 aut
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| 700 |
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|a Zhang, Shu-wen
|e verfasserin
|4 aut
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| 700 |
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|a Li, Min
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Hui-ji
|e verfasserin
|4 aut
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| 773 |
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8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 18(2006), 12 vom: 14. Dez., Seite 711-5
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
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|g volume:18
|g year:2006
|g number:12
|g day:14
|g month:12
|g pages:711-5
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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| 951 |
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|a AR
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|d 18
|j 2006
|e 12
|b 14
|c 12
|h 711-5
|