Characterization of DNA hybridization on partially aminated diamond by aromatic compounds

Here, we report a novel method of micropatterning oligonucleotides via aromatic groups as linkers on partially amino-terminated diamond and the inherence on subsequent hybridization. The covalent immobilization of probe oligonucleotides and characterization of immobilized probe oligonucleotides with...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 22(2006), 26 vom: 19. Dez., Seite 11245-50
1. Verfasser: Yang, Jung-Hoon (VerfasserIn)
Weitere Verfasser: Song, Kwang-Soup, Zhang, Guo-Jun, Degawa, Munenori, Sasaki, Yoshinori, Ohdomari, Iwao, Kawarada, Hiroshi
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't Carbocyanines Cross-Linking Reagents Oligonucleotides cyanine dye 5 Diamond 7782-40-3
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245 1 0 |a Characterization of DNA hybridization on partially aminated diamond by aromatic compounds 
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520 |a Here, we report a novel method of micropatterning oligonucleotides via aromatic groups as linkers on partially amino-terminated diamond and the inherence on subsequent hybridization. The covalent immobilization of probe oligonucleotides and characterization of immobilized probe oligonucleotides with carboxylic compounds were investigated by X-ray photoelectron spectroscopy (XPS). To confirm the effects of linker flexibility in a low amino group on diamond for probe oligonucleotides, three kinds of dicarboxylic compound--adipic acid, terephthalic acid, and trimesic acid--were used for immobilization of probe oligonucleotides, like linkers; and these oligonucleotides were hybridized with target oligonucleotides labeled with Cy 5 on the micropatterned diamond surface. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed 
650 4 |a Comparative Study 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Cross-Linking Reagents  |2 NLM 
650 7 |a Oligonucleotides  |2 NLM 
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700 1 |a Song, Kwang-Soup  |e verfasserin  |4 aut 
700 1 |a Zhang, Guo-Jun  |e verfasserin  |4 aut 
700 1 |a Degawa, Munenori  |e verfasserin  |4 aut 
700 1 |a Sasaki, Yoshinori  |e verfasserin  |4 aut 
700 1 |a Ohdomari, Iwao  |e verfasserin  |4 aut 
700 1 |a Kawarada, Hiroshi  |e verfasserin  |4 aut 
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