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|a pubmed24n0557.xml
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|a (DE-627)NLM167054910
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|a (NLM)17154611
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Yang, Jung-Hoon
|e verfasserin
|4 aut
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|a Characterization of DNA hybridization on partially aminated diamond by aromatic compounds
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|c 2006
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
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|2 rdacarrier
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|a Date Completed 26.01.2007
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|a Date Revised 12.12.2006
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|a published: Print
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|a Citation Status MEDLINE
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|a Here, we report a novel method of micropatterning oligonucleotides via aromatic groups as linkers on partially amino-terminated diamond and the inherence on subsequent hybridization. The covalent immobilization of probe oligonucleotides and characterization of immobilized probe oligonucleotides with carboxylic compounds were investigated by X-ray photoelectron spectroscopy (XPS). To confirm the effects of linker flexibility in a low amino group on diamond for probe oligonucleotides, three kinds of dicarboxylic compound--adipic acid, terephthalic acid, and trimesic acid--were used for immobilization of probe oligonucleotides, like linkers; and these oligonucleotides were hybridized with target oligonucleotides labeled with Cy 5 on the micropatterned diamond surface. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed
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|a Comparative Study
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Carbocyanines
|2 NLM
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|a Cross-Linking Reagents
|2 NLM
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|a Oligonucleotides
|2 NLM
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|a cyanine dye 5
|2 NLM
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|a Diamond
|2 NLM
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|a 7782-40-3
|2 NLM
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|a Song, Kwang-Soup
|e verfasserin
|4 aut
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|a Zhang, Guo-Jun
|e verfasserin
|4 aut
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|a Degawa, Munenori
|e verfasserin
|4 aut
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|a Sasaki, Yoshinori
|e verfasserin
|4 aut
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|a Ohdomari, Iwao
|e verfasserin
|4 aut
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|a Kawarada, Hiroshi
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 22(2006), 26 vom: 19. Dez., Seite 11245-50
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:22
|g year:2006
|g number:26
|g day:19
|g month:12
|g pages:11245-50
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|d 22
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|e 26
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|h 11245-50
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