Immobilization of enamel matrix derivate protein onto polypeptide multilayers. Comparative in situ measurements using ellipsometry, quartz crystal microbalance with dissipation, and dual-polarization interferometry

The buildup of biodegradable poly(L-glutamic acid) (PGA) and poly(L-lysine) (PLL) multilayers on silica and titanium surfaces and the immobilization of enamel matrix derivate (EMD) protein was followed by utilizing in situ ellipsometry, quartz crystal microbalance with dissipation, and dual-polariza...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 22(2006), 26 vom: 19. Dez., Seite 11065-71
1. Verfasser: Halthur, Tobias J (VerfasserIn)
Weitere Verfasser: Claesson, Per M, Elofsson, Ulla M
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't Coated Materials, Biocompatible Dental Enamel Proteins Dental Implants enamel matrix proteins Polylysine 25104-18-1 Polyglutamic Acid mehr... 25513-46-6 Silicon Dioxide 7631-86-9 Titanium D1JT611TNE
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100 1 |a Halthur, Tobias J  |e verfasserin  |4 aut 
245 1 0 |a Immobilization of enamel matrix derivate protein onto polypeptide multilayers. Comparative in situ measurements using ellipsometry, quartz crystal microbalance with dissipation, and dual-polarization interferometry 
264 1 |c 2006 
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500 |a Date Revised 21.11.2013 
500 |a published: Print 
500 |a Citation Status MEDLINE 
520 |a The buildup of biodegradable poly(L-glutamic acid) (PGA) and poly(L-lysine) (PLL) multilayers on silica and titanium surfaces and the immobilization of enamel matrix derivate (EMD) protein was followed by utilizing in situ ellipsometry, quartz crystal microbalance with dissipation, and dual-polarization interferometry (DPI). The use of the relatively new DPI technique validated earlier published ellipsometry measurements of the PLL-PGA polypeptide films. The hydrophobic aggregating EMD protein was successfully immobilized both on top of and within the multilayer structures at pH 5.0. DPI measurements further indicated that the immobilization of EMD is influenced by the flow pattern during adsorption. The formed polypeptide-EMD multilayer films are of interest since it is known that EMD is able to trigger cell response and induce biomineralization. The multilayer films thus have potential to be useful as bioactive and biodegradable coatings for future dental implants 
650 4 |a Comparative Study 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Dental Enamel Proteins  |2 NLM 
650 7 |a Dental Implants  |2 NLM 
650 7 |a enamel matrix proteins  |2 NLM 
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650 7 |a Titanium  |2 NLM 
650 7 |a D1JT611TNE  |2 NLM 
700 1 |a Claesson, Per M  |e verfasserin  |4 aut 
700 1 |a Elofsson, Ulla M  |e verfasserin  |4 aut 
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