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231223s2006 xx ||||| 00| ||eng c |
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|a pubmed24n0556.xml
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|a (DE-627)NLM166814970
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|a (NLM)17129059
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a White, Ryan J
|e verfasserin
|4 aut
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|a Ionic conductivity of the aqueous layer separating a lipid bilayer membrane and a glass support
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|c 2006
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 19.01.2007
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a The in-plane ionic conductivity of the approximately 1-nm-thick aqueous layer separating a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayer membrane and a glass support was investigated. The aqueous layer conductivity was measured by tip-dip deposition of a POPC bilayer onto the surface of a 20- to 75-microm-thick glass membrane containing a single conical-shaped nanopore and recording the current-voltage (i-V) behavior of the glass membrane nanopore/POPC bilayer structure. The steady-state current across the glass membrane passes through the nanopore (45-480 nm radius) and spreads radially outward within the aqueous layer between the glass support and bilayer. This aqueous layer corresponds to the dominant resistance of the glass membrane nanopore/POPC bilayer structure. Fluorescence recovery after photobleaching measurements using dye-labeled lipids verified that the POPC bilayer maintains a significant degree of fluidity on the glass membrane. The slopes of ohmic i-V curves yield an aqueous layer conductivity of (3 +/- 1) x 10(-3) Omega(-1) cm(-1) assuming a layer thickness of 1.0 nm. This conductivity is essentially independent of the concentration of KCl in the bulk solution (10-4 to 1 M) in contact with the membrane. The results indicate that the concentration and mobility of charge carriers in the aqueous layer between the glass support and bilayer are largely determined by the local structure of the glass/water/bilayer interface
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Lipid Bilayers
|2 NLM
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|a Membranes, Artificial
|2 NLM
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|a Phosphatidylcholines
|2 NLM
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|a Water
|2 NLM
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|a 059QF0KO0R
|2 NLM
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|a Potassium
|2 NLM
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|a RWP5GA015D
|2 NLM
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| 650 |
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|a 1-palmitoyl-2-oleoylphosphatidylcholine
|2 NLM
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| 650 |
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|a TE895536Y5
|2 NLM
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| 700 |
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|a Zhang, Bo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Daniel, Susan
|e verfasserin
|4 aut
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| 700 |
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|a Tang, John M
|e verfasserin
|4 aut
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| 700 |
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|a Ervin, Eric N
|e verfasserin
|4 aut
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| 700 |
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|a Cremer, Paul S
|e verfasserin
|4 aut
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| 700 |
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|a White, Henry S
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 22(2006), 25 vom: 05. Dez., Seite 10777-83
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:22
|g year:2006
|g number:25
|g day:05
|g month:12
|g pages:10777-83
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|a GBV_NLM
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