Detection of Cryptosporidium parvum oocysts in sediment and biosolids by immunomagnetic separation
A method for the detection of Cryptosporidium parvum oocysts in sediment and wastewater biosolids has been developed using immunomagnetic separation kits that were designed for use with water. This method requires no pretreatment of the sediment or biosolids samples before the commercial kit applica...
| Veröffentlicht in: | Water environment research : a research publication of the Water Environment Federation. - 1998. - 78(2006), 9 vom: 20. Sept., Seite 1013-6 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2006
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| Zugriff auf das übergeordnete Werk: | Water environment research : a research publication of the Water Environment Federation |
| Schlagworte: | Journal Article Sewage |
| Zusammenfassung: | A method for the detection of Cryptosporidium parvum oocysts in sediment and wastewater biosolids has been developed using immunomagnetic separation kits that were designed for use with water. This method requires no pretreatment of the sediment or biosolids samples before the commercial kit application. Oocyst recovery efficiencies from sediment and biosolids using the modified Dynal (Lake Success, New York) and Crypto-Scan commercial methods (Immucell Corporation, Portland, Maine) ranged from 20 to 60%. While the sensitivity of the method is dependent on the amount of sediment processed and the equivalent volume examined under the microscope, it was able to detect 0.48 oocysts per gram dry weight sediment. Using this method, Cryptosporidium parvum oocysts were found at levels as high as 97 oocysts/g of primary biosolids and at levels up to 4 oocysts/g in polluted sediment |
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| Beschreibung: | Date Completed 17.01.2007 Date Revised 22.09.2019 published: Print Citation Status MEDLINE |
| ISSN: | 1554-7531 |